AMPK beta subunits display isoform specific affinities for carbohydrates

FEBS Letters
Ann KoayPaul R Gooley

Abstract

AMP-activated protein kinase (AMPK) is a heterotrimer of catalytic (alpha) and regulatory (beta and gamma) subunits with at least two isoforms for each subunit. AMPK beta1 is widely expressed whilst AMPK beta2 is highly expressed in muscle and both beta isoforms contain a mid-molecule carbohydrate-binding module (beta-CBM). Here we show that beta2-CBM has evolved to contain a Thr insertion and increased affinity for glycogen mimetics with a preference for oligosaccharides containing a single alpha-1,6 branched residue. Deletion of Thr-101 reduces affinity for single alpha-1,6 branched oligosaccharides by 3-fold, while insertion of this residue into the equivalent position in the beta1-CBM sequence increases affinity by 3-fold, confirming the functional importance of this residue.

References

Sep 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M D AlonsoW J Whelan
Sep 26, 1997·The Journal of Biological Chemistry·K I MitchelhillB E Kemp
Apr 13, 2002·Current Molecular Medicine·Peter J Roach
Dec 19, 2002·American Journal of Physiology. Endocrinology and Metabolism·Jorgen F P WojtaszewskiErik A Richter
Jan 28, 2003·Biochemical Society Transactions·B E KempL A Witters
May 16, 2003·Current Biology : CB·Galina PolekhinaDavid Stapleton
Oct 8, 2003·Systematic Biology·Stéphane Guindon, Olivier Gascuel
Jun 25, 2004·The Biochemical Journal·Alisdair B BorastonGideon J Davies
Feb 8, 2005·The Journal of Biological Chemistry·Tristan J IseliDavid Stapleton
May 12, 2005·American Journal of Physiology. Endocrinology and Metabolism·Hideyuki SakodaTomoichiro Asano
Jun 2, 2005·Journal of the American Chemical Society·Paul Schanda, Bernhard Brutscher
Oct 12, 2005·Structure·Galina PolekhinaMichael W Parker
Oct 14, 2006·The Journal of Physiology·J B Birk, J F P Wojtaszewski
Mar 29, 2008·Briefings in Bioinformatics·Kazutaka Katoh, Hiroyuki Toh
Nov 18, 2008·Biochemical and Biophysical Research Communications·Ji-Hye ChoiKwan-Hwa Park
Aug 18, 2009·The FEBS Journal·Camilla ChristiansenBirte Svensson
Sep 2, 2009·International Journal of Biological Macromolecules·Je-Hoon RyuDavid Stapleton

❮ Previous
Next ❯

Citations

Mar 26, 2013·Proteome Science·David StapletonGlendon Parker
Feb 24, 2016·Acta Pharmaceutica Sinica. B·David Grahame Hardie
Dec 9, 2015·Trends in Plant Science·Shane EmanuellePaul R Gooley
Jan 13, 2015·Extremophiles : Life Under Extreme Conditions·Il-Nam OhKwan-Hwa Park
Aug 25, 2015·Molecular Aspects of Medicine·Joohun HaJoungmok Kim
Oct 30, 2015·Journal of Medicinal Chemistry·Marie MiglianicoDietbert Neumann
Jun 7, 2014·Frontiers in Plant Science·Alejandra Avila-CastañedaPatricia Coello
Nov 22, 2014·Cell Research·Xiaodan LiKarsten Melcher
May 4, 2016·Cellular and Molecular Life Sciences : CMLS·Marie Sofie MøllerBirte Svensson
Dec 2, 2017·Chembiochem : a European Journal of Chemical Biology·Jesse I MobbsPaul R Gooley
Jul 14, 2018·The Plant Journal : for Cell and Molecular Biology·Ana Ruiz-GayossoPatricia Coello
Mar 15, 2019·Journal of Experimental Botany·Muhammed Jamsheer KAshverya Laxmi
Mar 8, 2012·American Journal of Physiology. Endocrinology and Metabolism·Andrew PhilpKeith Baar
Aug 27, 2019·Journal of Integrative Plant Biology·Wei WangPeisong Hu
Apr 22, 2016·American Journal of Physiology. Cell Physiology·Hongyang XuRobyn M Murphy
Jun 1, 2013·The FEBS Journal·Pascual SanzMaria Adelaida Garcia-Gimeno
Jul 23, 2018·Advances in Nutrition·Long HeXi Ma
Nov 15, 2018·International Journal of Molecular Sciences·Natalia A VilchinskayaBoris S Shenkman
Mar 21, 2015·The Journal of Biological Chemistry·Yvonne OligschlaegerDietbert Neumann
Oct 31, 2018·International Journal of Molecular Sciences·Natalie R JanzenNolan J Hoffman
Mar 5, 2015·The Plant Journal : for Cell and Molecular Biology·Shane EmanuelleDavid I Stapleton
Mar 12, 2021·Journal of Experimental Botany·Muhammed Jamsheer KVibha Srivastava
Sep 3, 2021·The EMBO Journal·Mengqiu YuanPing Gao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.