AMPK Hyper-Activation Alters Fatty Acids Metabolism and Impairs Invasiveness of Trophoblasts in Preeclampsia

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Xiaotao YangPhilip N Baker

Abstract

Preeclampsia (PE) has long been assumed to be an ischemic disease of the placenta, although there is limited evidence as to how the ischemia impacts on the placenta. AMP-activated protein kinase (AMPK) is a key regulator of cellular energy metabolism and plays an important role in a variety of ischemic diseases by enhancing energy production. The present study investigated placental metabolism in PE, and the role of AMPK in regulating trophoblast function. placentas from normal and PE complicated pregnancies were subjected to GC-MS to identify fatty acids (FA) metabolic fingerprints, and total FA oxidation was assessed by malondialdehyde (MDA) measurement. The AMPK-ACC signaling pathway was assessed by q-PCR and Western Blotting. HTR8/SVneo trophoblast cultures were exposed to different oxygenation conditions to establish an in vitro PE cell model; further analysis by GC-MS for metabolite profiling was then undertaken. Trophoblasts invasion was assessed by a matrigel transwell assay in the presence/absence of AMPK expression and after manipulations of AMPK activity, and then further validated by human villi outgrowth experiments. AMPK phosphorylation and MDA production were significantly elevated in placentas from pregnancies c...Continue Reading

Citations

May 13, 2020·International Journal of Molecular Sciences·Akitoshi NakashimaShigeru Saito
Jan 8, 2020·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Li-Yuan WangJun Ma
Jan 30, 2020·International Journal of Molecular Sciences·Stacey J ElleryRod J Snow
Feb 3, 2021·The FEBS Journal·Mingyu HuChao Tong
Jun 3, 2021·Placenta·Emma Siragher, Amanda N Sferruzzi-Perri
Apr 5, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zhixiong MeiNi Deng

❮ Previous
Next ❯

Related Concepts

Related Feeds

Attention Disorders

Attention is involved in all cognitive activities, and attention disorders are reported in patients with various neurological diseases. Here are the latest discoveries pertaining to attention disorders.