AMPKα2 deficiency exacerbates long-term PM2.5 exposure-induced lung injury and cardiac dysfunction

Free Radical Biology & Medicine
Hongyun WangZhongbing Lu

Abstract

Previous studies have demonstrated that long-term exposure to fine particulate matter (PM2.5) increases the risk of respiratory and cardiovascular diseases. As a metabolic sensor, AMP-activated protein kinase (AMPK) is a promising target for cardiovascular disease. However, the impact of AMPK on the adverse health effects of PM2.5 has not been investigated. In this study, we exposed wild-type (WT) and AMPKα2-/- mice to either airborne PM2.5 (mean daily concentration ~64 µg/m3) or filtered air for 6 months through a whole-body exposure system. After exposure, AMPKα2-/- mice developed severe lung injury and left ventricular dysfunction. In the PM2.5-exposed lungs and hearts, loss of AMPKα2 resulted in higher levels of fibrotic genes, more collagen deposition, lower levels of peroxiredoxin 5 (Prdx5), and greater induction of oxidative stress and inflammation than observed in the lungs and hearts of WT mice. In PM2.5-exposed BEAS-2B and H9C2 cells, inhibition of AMPK activity significantly decreased cell viability and Prdx5 expression, and increased the intracellular ROS and p-NF-κB levels. Collectively, our results provide the first direct evidence that AMPK has a marked protective effect on the adverse health effects induced by l...Continue Reading

Citations

Jul 26, 2019·Journal of Toxicology and Environmental Health. Part a·Wonhwa LeeJong-Sup Bae
Aug 14, 2019·International Journal of Environmental Health Research·Wonhwa LeeJong-Sup Bae
Jun 20, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Amina KunovacJohn M Hollander
Sep 15, 2020·Free Radical Research·Zhen-Zhong WuQi-Zhu Tang
Sep 12, 2020·Journal of Ethnopharmacology·Rentsen BadamjavFang Li
Mar 7, 2021·International Journal of Molecular Sciences·Omar HahadThomas Münzel
Jan 30, 2021·American Journal of Physiology. Heart and Circulatory Physiology·Agnes Maria LedererNandu Goswami
Apr 14, 2021·The Science of the Total Environment·Zenghua QiZongwei Cai
Apr 17, 2021·Environmental Toxicology and Pharmacology·Ning ZhangGang Chen
Jun 21, 2021·Stem Cell Research & Therapy·Junling GaoZhongbing Lu
Mar 7, 2020·Genomics, Proteomics & Bioinformatics·Xiao HanWenjun Ding
Aug 9, 2021·Environmental Science and Pollution Research International·Jinjin JiangShibin Ding

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiovascular Inflammation

Inflammation plays a significant role in the development of cardiovascular diseases, an understanding of these endogenous processes is critical for evaluating the risks and potential treatment strategies. Discover the latest research on cardiovascular inflammation here.

Cardiovascular Disease Pathophysiology

Cardiovascular disease involves several different processes that contribute to the pathological mechanism, including hyperglycemia, inflammation, atherosclerosis, hypertension and more. Vasculature stability plays a critical role in the development of the disease. Discover the latest research on cardiovascular disease pathophysiology here.