Amplification and overexpression of PPFIA1, a putative 11q13 invasion suppressor gene, in head and neck squamous cell carcinoma

Genes, Chromosomes & Cancer
Kaia Davis TanPatrick Tan

Abstract

Chromosomal amplifications of the 11q13 genomic region are frequent in head and neck squamous cell carcinoma (HNSCC). To identify novel 11q13 amplification targets, we integrated high-resolution array-based comparative genomic hybridization and Affymetrix gene-expression profiling of eight HNSCC cell lines. We found that PPFIA1 was the highest upregulated gene in the 11q13 amplicon of HNSCC cell lines when compared with HNSCC lines without 11q13 amplification and confirmed the upregulation of PPFIA1 in primary HNSCCs by real-time PCR. Using siRNA knockdown, we investigated PPFIA1 function in three HNSCC lines using both in vitro invasion assays and wound-healing assays. Surprisingly, we found that cancer cells become more invasive when the PPFIA1 protein levels were reduced, suggesting that PPFIA1 may act as an invasion inhibitor in HNSCC. This unexpected result suggests that the 11q13 amplicon may comprise both positive and negative regulators involved in HNSCC. Our study is the first to evaluate the role of PPFIA1 in head and neck carcinogenesis and suggests a potential link between PPFIA1 activity and cell-extracellular matrix interactions. This article contains supplementary material available via the Internet at http://www...Continue Reading

References

Apr 1, 1995·Genes, Chromosomes & Cancer·C M LeseS M Gollin
Jul 1, 1995·Archives of Otolaryngology--head & Neck Surgery·S D MeredithM E Williams
Aug 1, 1994·Japanese Journal of Cancer Research : Gann·K TakenagaS Sakiyama
Jan 21, 1993·The New England Journal of Medicine·E E VokesW K Hong
Feb 21, 1998·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·D MullerJ Abecassis
Jun 23, 1998·The Journal of Biological Chemistry·C Serra-PagèsM Streuli
Aug 10, 1999·Nature·W C HahnR A Weinberg
Oct 20, 1999·The Annals of Otology, Rhinology, and Laryngology·S AlaviK E Blackwell
Nov 24, 1999·Head & Neck·F BruninJ Rodriguez
Aug 10, 2000·The American Journal of Pathology·U BockmühlI Petersen
Sep 13, 2001·Proceedings of the National Academy of Sciences of the United States of America·P DuesbergR Hehlmann
Jan 5, 2002·The New England Journal of Medicine·A ForastiereD Sidransky
Nov 26, 2002·Proceedings of the National Academy of Sciences of the United States of America·Martin A NowakChristoph Lengauer
Mar 13, 2003·Breast Cancer Research : BCR·Olle StålLars Erik Rutqvist
Apr 25, 2003·Trends in Genetics : TIG·Marjori A MatzkeAntonius J M Matzke
Feb 26, 2004·Nature Genetics·Adrian S IshkanianWan L Lam
Apr 8, 2004·Neoplasia : an International Journal for Oncology Research·Daniel R RhodesArul M Chinnaiyan
Sep 17, 2004·The Journal of Pathology·Yann-Jang ChenKuo-Wei Chang
Oct 2, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Volkert B WreesmannBhuvanesh Singh
Jan 22, 2005·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun
Mar 12, 2005·CA: a Cancer Journal for Clinicians·D Max ParkinPaola Pisani
Oct 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Gitta GoesselOliver G Opitz
Mar 22, 2006·Oncogene·K E KnudsenE S Knudsen
Sep 16, 2006·The Journal of Biological Chemistry·Motoko UnokiCurtis C Harris

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Citations

Mar 5, 2013·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·Marco BlessmannAlexander Gröbe
Jun 3, 2014·World Journal of Surgical Oncology·Eun Ji ChoiSu Young Kim
Nov 11, 2011·Genes, Chromosomes & Cancer·Srikant AmbatipudiManoj B Mahimkar
Feb 13, 2010·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Kolja FreierChristel Herold-Mende
Sep 30, 2009·Genes, Chromosomes & Cancer·Ana-Maria DancauRonald Simon
Jul 10, 2012·Genomics, Proteomics & Bioinformatics·Subhash J JakhesaraChaitanya G Joshi
Nov 19, 2020·British Journal of Cancer·Nikita M NovikovEvgeny V Denisov
Oct 17, 2021·Oncogene·Henna PehkonenOuti Monni

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