Amplification and restriction endonuclease digestion of a large fragment of genes coding for rRNA as a rapid method for discrimination of closely related pathogenic bacteria.

Journal of Clinical Microbiology
A IbrahimAnders Sjöstedt

Abstract

By use of primers specific to conserved regions of the rRNA gene cluster, a discrete amplicon of approximately 5 kb was amplified by PCR from all 21 bacterial genera investigated. Subsequent endonuclease digestion of the PCR product with HaeIII distinguished between the three species of the human pathogen Francisella spp. on the one hand and four clinically relevant genomic groups of Acinetobacter spp. on the other hand. The described technique has the potential as a rapid method for discriminating between closely related species that are of clinical importance.

References

Jun 1, 1992·Journal of Bacteriology·J J SchwartzI Schwartz
Oct 1, 1992·Journal of Clinical Microbiology·P Gerner-Smidt
Feb 1, 1991·Journal of Clinical Microbiology·P Gerner-SmidtJ Ursing
May 3, 1990·Nature·S J GiovannoniK G Field
Jul 1, 1989·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·I Tjernberg, J Ursing
Jun 1, 1982·Journal of Clinical Microbiology·D J BrennerA G Steigerwalt

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Citations

Jan 11, 2005·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Anders JohanssonAnders Sjöstedt
Oct 5, 2002·Clinical Microbiology Reviews·Jill EllisRichard W Titball
Feb 8, 2003·Journal of Clinical Microbiology·Laurent SchlegelAnne Bouvet

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