PMID: 7007868Nov 20, 1980Paper

Amplification by enzymatic cycling

Molecular and Cellular Biochemistry
O H Lowry

Abstract

Enzymatic cycling provides a methodology for virtually unlimited amplification of analytical sensitivity. The most widely applicable cycling systems are those for NAD and NADP, since these can be used to increase the sensitivity of methods for a host of other substances. However, cycling systems for ATP plus ADP, GTP + GDP, glutathione and coenzyme A have also proven to be very useful. A total of 19 cycling procedures are described in greater or lesser detail. Some of these are capable of amplification rates in excess of 20,000 per hour in a single cycling step (20,000 x 20,000 with two one hour cycling steps). Advantages, disadvantages, limitations and other practical considerations are stressed, as well as the means for coupling the cycling systems to assays for other substances.

Citations

Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·C R Mateo, A Douhal
May 31, 2011·Analytical Biochemistry·Shin-ichi SakasegawaHaruhiko Sakuraba
Mar 1, 1987·Analytical Biochemistry·M M ChiO H Lowry
Jul 22, 2019·Diagnostics·Kanako IhaEtsuro Ito
Mar 1, 2016·Current Protocols in Plant Biology·Camille Bénard, Yves Gibon
Jan 1, 1983·Transactions of the New York Academy of Sciences·O H Lowry

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