Amplification-free digital gene expression profiling from minute cell quantities.

Nature Methods
Fatih OzsolakDaniel A Haber

Abstract

Generating reliable expression profiles from minute cell quantities is critical for scientific discovery and potential clinical applications. Here we present low-quantity digital gene expression (LQ-DGE), an amplification-free approach involving capture of poly(A)(+) RNAs from cellular lysates onto poly(dT)-coated sequencing surfaces, followed by on-surface reverse transcription and sequencing. We applied LQ-DGE to profile malignant and nonmalignant mouse and human cells, demonstrating its quantitative power and potential applicability to archival specimens.

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Citations

Jan 25, 2012·Cellular and Molecular Neurobiology·Ruby KwongMichal Janitz
Apr 1, 2011·Nature Methods·Fuchou TangM Azim Surani
Nov 22, 2011·Nature Methods·Teemu KiviojaJussi Taipale
Oct 29, 2013·Nature Neuroscience·Suzanne E HickmanJoseph El Khoury
Dec 31, 2010·Nature Reviews. Genetics·Fatih Ozsolak, Patrice M Milos
Jan 11, 2012·Proceedings of the National Academy of Sciences of the United States of America·Katsuyuki ShiroguchiX Sunney Xie
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Dec 12, 2012·International Journal of Plant Genomics·Santosh KumarSylvie Cloutier
Sep 8, 2011·Nature Reviews. Genetics·Jeffrey A Martin, Zhong Wang
Apr 4, 2012·Expert Opinion on Drug Discovery·Fatih Ozsolak
Sep 6, 2013·Journal of Molecular Signaling·Kristine Raaby JakobsenAnders Lade Nielsen
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Feb 26, 2011·Genome Biology·John F Thompson, Patrice M Milos
Jul 11, 2018·Genome Biology·Yihan Wan, Daniel R Larson

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