Amplification of 1-deoxy-D-xyluose 5-phosphate (DXP) synthase level increases coenzyme Q10 production in recombinant Escherichia coli

Applied Microbiology and Biotechnology
Soo-Jung KimJin-Ho Seo

Abstract

For the enhancement of coenzyme Q(10) (CoQ(10)) production, 1-deoxy-D-xylulose 5-phosphate (DXP) synthase of Pseudomonas aeruginosa was constitutively coexpressed in a recombinant Escherichia coli strain, which harbors the ddsA gene from Gluconobacter suboxydans encoding decaprenyl diphosphate synthase. It was found that the expression of the ddsA gene caused depletion of the isopentenyl diphosphate (IPP) pool in E. coli. Amplification of DXP synthase level by installing P. aeruginosa DXP synthase restored the diminished IPP pool and concomitantly resulted in approximately a twofold increase in relative content and productivity of CoQ(10). Maximum CoQ(10) concentration of 46.1 mg l(-1) was achieved from glucose-limited fed-batch cultivation of the recombinant E. coli strain simultaneously harboring the ddsA and dxs genes.

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Citations

Dec 17, 2009·Applied Microbiology and Biotechnology·Marimuthu JeyaJung-Kul Lee
Jun 9, 2014·Journal of Industrial Microbiology & Biotechnology·Wen XuJiankang Liu
Nov 19, 2014·Microbial Cell Factories·Jianming Yang, Lizhong Guo
Oct 12, 2011·Metabolic Engineering·Corinne P CluisVincent J J Martin
Sep 17, 2015·Applied Microbiology and Biotechnology·Zheng GuanWim J Quax
Jul 19, 2015·Journal of Industrial Microbiology & Biotechnology·Irene MartínezAlvaro Díaz-Barrera
Oct 16, 2007·Trends in Biotechnology·Corinne P CluisVincent J J Martin
Oct 13, 2016·World Journal of Microbiology & Biotechnology·Wen XuYang Wang
Mar 4, 2017·Microbial Cell Factories·Sean Qiu En LeeEe Sin Chen
Mar 28, 2019·Bioprocess and Biosystems Engineering·Irene MartínezAlvaro Díaz-Barrera

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