Amplification of arachidonic acid metabolism in rat liver cells (the C-9 cell-line) by tosyl-L-phenylalanine chloromethyl ketone and phenylmethyl-sulfonyl fluoride

Prostaglandins
L Levine

Abstract

In the presence of the protease inhibitors phenylmethyl-sulfonyl fluoride and N-tosyl-L-phenylalanine chloromethyl ketone, prostacyclin (PGI2) production by rat liver cells treated with epidermal growth factor, platelet-activating factor, 12-0-tetradecanoylphorbol-13-acetate (TPA), and TPA-type tumor promoters (teleocidin and aplysiatoxin) or 1-oleoyl-2-acetylglycerol is amplified. The PGI2 production stimulated by thapsigargin or exogenous arachidonic acid is not amplified. N-Tosyl-L-phenylalanine chloromethyl ketone also amplifies TPA's release of radioactivity from cells isotopically labeled with [3H]arachidonic acid. Indomethacin inhibits the amplification of PGI2 production but no the release of radioactivity. The presence of the protease inhibitors is not required for the amplification of PGI2 production. Prior incubation of the cells with these inhibitors, followed by their removal, still results in amplified PGI2 production by cells subsequently treated with TPA, 1-oleoyl-2-acetylglycerol, or platelet-activating factor but not that stimulated by exogenous arachidonic acid. While phenylmethyl-sulfonyl fluoride's amplification of PGI2 production by cells treated with TPA was blocked by prior incubation with TPA for 20 h, ...Continue Reading

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Citations

Jul 4, 2003·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Mira KojouharovaUNKNOWN Bulgarian Hib Study Team
Sep 29, 2006·The Pediatric Infectious Disease Journal·Jennifer C MoïsiJames P Watt

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