Amplification of JC virus regulatory DNA sequences from cerebrospinal fluid: diagnostic value for progressive multifocal leukoencephalopathy

Archives of Virology
C SugimotoY Yogo

Abstract

Progressive multifocal leukoencephalopathy (PML) is a fatal demyelinating disease in the central nervous system caused by a ubiquitous human polyomavirus designated as JC virus (JCV). PML affects individuals with decreased immune competence and is now one of the common opportunistic infections in patients with AIDS. JCV DNAs in the brain of PML patients contain various PML-type regulatory regions that were generated from the archetypal regulatory region during persistence. Recently, many studies have suggested that detection of JCV DNA from the cerebrospinal fluid (CSF) may offer a tool for diagnosing PML. However, in all of these studies, coding sequences within the T antigen or capsid protein gene have been targeted for amplification. To amplify the JCV regulatory region, we established a nested PCR that could efficiently amplify the regulatory region from most JCV subtypes prevalent in the world. Using this PCR, we amplified JCV regulatory regions from the CSF samples from 4 patients strongly suspected of PML, whereas amplification was negative from 80 CSF samples from patients without PML. Sequencing of the amplified fragments revealed that they had unique deletions and/or duplications. Furthermore, in 3 PML patients, we an...Continue Reading

Citations

Aug 10, 2005·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Adrienne L McNeesJanet S Butel
May 29, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Darío García De ViedmaEmilio Bouza
Mar 25, 2011·Journal of Clinical Microbiology·Damien RouxJerôme Legoff
Oct 31, 2006·Journal of Clinical Pathology·S Love
Nov 13, 2008·Radiographics : a Review Publication of the Radiological Society of North America, Inc·Alice B SmithElisabeth J Rushing
Mar 12, 2003·Journal of Medical Virology·Gunn Eli Kimo JørgensenJohn Inge Johnsen
Oct 15, 2013·Journal of Medical Virology·Danijela KaralicTanja Jovanovic
Jul 5, 2006·Therapeutic Apheresis and Dialysis : Official Peer-reviewed Journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy·Hiroto MatsudaTakao Saruta
Mar 21, 2008·Pediatrics International : Official Journal of the Japan Pediatric Society·Yukiharu YasudaShunichi Kato
Nov 1, 2005·Clinical Neurology and Neurosurgery·Akiko KimuraToshihide Kumamoto
Sep 23, 2006·Proteomics·Veerasamy Ravichandran, Eugene O Major
Mar 2, 2006·Arthritis and Rheumatism·Kenji ItohMorito Sumiya
Oct 4, 2020·Biochemical and Biophysical Research Communications·Emiko ShimboYoh-Ichi Tagawa

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