Amplification of Mycobacterium tuberculosis from peripheral blood.

Journal of Clinical Microbiology
A RolfsH Lode

Abstract

To determine the value of the PCR assay of lymphomonocytic blood cells for the diagnosis of pulmonary tuberculosis, we compared, in a prospective study, PCR results with results of conventional diagnostic methods of Mycobacterium tuberculosis detection. Clinical investigators who were unaware of the PCR data classified 162 immunocompetent patients, who had been hospitalized because of suspected pulmonary tuberculosis, in accordance with the recommendations of the American Thoracic Society (ATS). By testing blood samples from these patients for mycobacterial DNA with three different PCR systems, we were able to demonstrate that nonquantitative PCR of peripheral blood leukocytes is of little value for the specific diagnosis of pulmonary tuberculosis. However, semiquantitative PCR assays might have some significance since we found an increasing level of mycobacterial DNA copies in blood from patients classified in ATS groups 2 to 5.

References

May 1, 1990·The Journal of Infectious Diseases·K D EisenachJ T Crawford
Jun 1, 1990·Journal of Clinical Microbiology·P W HermansJ D van Embden
Sep 1, 1990·The American Review of Respiratory Disease
Jan 1, 1995·Journal of Clinical Microbiology·J BeigeA Rolfs
Mar 1, 1994·Journal of Clinical Microbiology·L F KoxA H Kolk

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Citations

Jan 11, 2005·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·I C ShamputaF Portaels
Dec 20, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Sonia H MontenegroRichard A Oberhelman
Dec 14, 2011·Applied Microbiology and Biotechnology·Sasikala SivagnanamRajamanickam Chellam
May 17, 2013·Journal of Clinical Microbiology·Padmapriya P BanadaDavid Alland
Oct 8, 1997·Journal of Clinical Microbiology·M S HughesS D Neill

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