Amplification of the prolactin receptor gene in mammary lobular neoplasia

Breast Cancer Research and Treatment
Danh Tran-ThanhSusan J Done

Abstract

The identification of lobular carcinoma in situ (LCIS) in a patient's specimen confers an appreciable increased risk of development of future invasive mammary carcinoma. However, the study of LCIS presents a challenge as it is usually only recognized in fixed specimens. Recent advances in high throughput genomics have made possible comprehensive copy number analysis of lesions such as this. Using array comparative genomic hybridization (aCGH), we characterized eight cases of lobular carcinoma (four invasive and four non-invasive) from microdissected samples of archival specimens and validated our results by quantitative real-time PCR (qRT-PCR). Immunohistochemistry (IHC) was performed on an independent set of 80 in situ ductal (DCIS) and lobular breast lesions to confirm our results. Amplification of the prolactin receptor gene (PRLr) was identified in 4/4 cases of LCIS by aCGH. We confirmed this amplification by qRT-PCR and demonstrated PRLr expression in 29/40 (73%) cases of lobular neoplasia by IHC. Amplification of PRLr was neither detected in 10 cases of DCIS nor in 5 areas of normal breast tissue by qRT-PCR and only 14/40 (35%) cases of DCIS showed PRLr expression by IHC (P = 0.0008). Our study suggests the prolactin rece...Continue Reading

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Citations

Sep 4, 2010·Breast Cancer Research and Treatment·Alejandro Gru, D Craig Allred
Jun 5, 2015·Breast Cancer Research : BCR·Greg J LoganPriscilla F McAuliffe
Oct 16, 2012·International Journal of Oncology·Lin HouDong-Xu Liu
Sep 9, 2020·Expert Opinion on Therapeutic Targets·Antonela S AsadMarianela Candolfi
Aug 7, 2021·PloS One·Amira AlkharusiFahad Zadjali

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