PMID: 11911586Mar 26, 2002Paper

Amplification of 'variola virus-specific' sequences in German cowpox virus isolates

Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health
Hermann MeyerM Pfeffer

Abstract

In 1995 a polymerase chain reaction (PCR) protocol describing the specific detection of variola virus, the causative agent of smallpox, was published by Knight and others. Virulent variola major strains could be differentiated from less virulent variola minor strains because of the distinct amplicon sizes. Here, we applied this PCR protocol to DNA from various orthopoxvirus isolates. There was no amplification with the orthopoxvirus species vaccinia, monkeypox, mousepox, or camelpox viruses. However, amplification was observed in six out of 15 cowpox virus strains investigated. The size of the amplicons corresponded exactly with the size described for variola minor strains and the nucleotide sequence identity accounted for 97%. Findings are discussed with respect to the evolution of orthopoxvirus species assuming that variola virus most probably stems from a rodent-transmitted cowpox virus-like progenitor.

References

Jan 1, 1992·Archives of Virology·J NaidooC J Gaskell
May 1, 1985·Virology·J J Esposito, J C Knight
Aug 1, 1995·Journal of Clinical Microbiology·S L RoppJ J Esposito
Aug 1, 1995·Virology·R F MassungJ J Esposito
Mar 1, 1996·Veterinary Microbiology·M PfefferO R Kaaden
Oct 21, 1998·Journal of Virological Methods·H NeubauerH Meyer
May 5, 1999·Archives of Virology·H MeyerM Pfeffer
Jan 20, 2000·Virology·S N ShchelkunovJ J Esposito

❮ Previous
Next ❯

Citations

Mar 17, 2004·Journal of Virological Methods·David PulfordDavid Ulaeto
Aug 9, 2003·Journal of Clinical Microbiology·M Sofi IbrahimPeter B Jahrling
May 8, 2004·Journal of Clinical Microbiology·Victoria A OlsonHermann Meyer
Apr 28, 2004·Emerging Infectious Diseases·Jeannette GuarnerUNKNOWN Veterinary Monkeypox Virus Working Group
Sep 4, 2007·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Andreas NitscheGeorg Pauli
May 5, 2006·Biological Reviews of the Cambridge Philosophical Society·Jessica M C Pearce-Duvet
Jan 18, 2005·Molecular and Cellular Probes·S N ShchelkunovI V Babkin
Apr 26, 2005·Journal of Virological Methods·Andreas NitscheGeorg Pauli
Oct 11, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Malachy Ifeanyi OkekeTerje Traavik
Mar 17, 2004·Current Opinion in Infectious Diseases·Sue Lewis-Jones
Jun 6, 2009·Vector Borne and Zoonotic Diseases·Sandra EssbauerMartin Pfeffer
Dec 13, 2006·Bulletin of Experimental Biology and Medicine·I V VinogradovE I Ryabchikova
Jun 23, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·David A KuleshGeorge V Ludwig
Mar 3, 2007·Clinical Chemistry·Natale ScaramozzinoDaniel Garin
Jan 24, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anindya DasguptaKlaus Früh
Jul 29, 2006·Science·Joseph J EspositoRobert M Wohlhueter

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.