Amplified fluorescence detection of mercury(II) ions (Hg2+) using target-induced DNAzyme cascade with catalytic and molecular beacons

The Analyst
Lin QiYayan Wu

Abstract

In this work, a fluorescent sensing strategy was developed for the detection of mercury(II) ions (Hg(2+)) in aqueous solution with excellent sensitivity and selectivity using a target-induced DNAzyme cascade with catalytic and molecular beacons (CAMB). In order to construct the biosensor, a Mg(2+)-dependent DNAzyme was elaborately designed and artificially split into two separate oligonucleotide fragments. In the presence of Hg(2+), the specific thymine-Hg(2+)-thymine (T-Hg(2+)-T) interaction induced the two fragments to produce the activated Mg(2+)-dependent DNAzyme, which would hybridize with a hairpin-structured MB substrate to form the CAMB system. Eventually, each target-induced activated DNAzyme could catalyze the cleavage of many MB substrates through true enzymatic multiple turnovers. This would significantly enhance the sensitivity of the Hg(2+) sensing system and push the detection limit down to 0.2 nM within a 20 min assay time, much lower than those of most previously reported fluorescence assays. Owning to the strong coordination of Hg(2+) to the T-T mismatched pairs, this proposed sensing system exhibited excellent selectivity for Hg(2+) detection, even in the presence of 100 times of other interferential metal io...Continue Reading

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Citations

Dec 24, 2013·Inorganic Chemistry·Yu Xiang, Yi Lu
May 17, 2014·Current Opinion in Biotechnology·Ki Soo Park, Hyun Gyu Park
Nov 10, 2015·Chemical Reviews·Yongxi ZhaoChunhai Fan
Oct 14, 2014·Journal of Colloid and Interface Science·Wentai WangQin Li
Oct 23, 2014·Chemical Communications : Chem Comm·Liang GongRu-Qin Yu
Jan 24, 2014·Analytical Chemistry·Chia-Wen LienHuan-Tsung Chang
Jul 23, 2013·Chemical Communications : Chem Comm·Fenglei GaoHuangxian Ju
Dec 4, 2020·Luminescence : the Journal of Biological and Chemical Luminescence·Pengfei FanJinquan Liu

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