Amplified HMGA2 promotes cell growth by regulating Akt pathway in AML

Journal of Cancer Research and Clinical Oncology
Li TanHuo Tan

Abstract

The aim of this study was to investigate how the amplification of HMGA2 contributes to acute myeloid leukemia (AML) cell proliferation. The amplification and expression of HMGA2 were examined by FISH, qRT-PCR and Western blot in AML cases. The effect of HMGA2 knockdown on cell proliferation was analyzed with XTT, colony-forming assays and BrdUrd incorporation assays. The effects of HMGA2 knockdown on cell cycle were studied by flow cytometry analysis. The progression of AML cells in vivo was examined by the xenografted tumor model. The interaction between Akt pathway and HMGA2 was examined by Western blot. HMGA2 is amplified in AML, and the levels of HMGA2 messenger RNA (mRNA) and protein expressed in AML cells were significantly higher than those in normal cells, which may be related to NR and prognosis of AML patients. Reduction in HMGA2 expression in AML cells inhibited cell proliferation through a decrease in the protein expression of pAkt and pmTOR, compared with control cells. HMGA2 is predominantly amplified and expressed in AML cells, and that aberrant expression of HMGA2 induces AML cell proliferation through the PI3K/Akt/mTOR signaling pathway. Inhibition of HMGA2 expression represents an attractive target for AML the...Continue Reading

References

Jan 1, 1996·Progress in Nucleic Acid Research and Molecular Biology·M Bustin, R Reeves
Apr 29, 1998·Nucleic Acids Research·F MantovaniG Manfioletti
Jun 19, 2001·Biochimica Et Biophysica Acta·R Reeves, L Beckerbauer
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 5, 2004·BMC Molecular Biology·Richard J Fish, Egbert K O Kruithof
Jun 13, 2006·Cancer Cell·Monica FedeleAlfredo Fusco
Nov 28, 2006·Lancet·Elihu Estey, Hartmut Döhner
Sep 6, 2007·Cancer·Farhad RavandiHagop M Kantarjian
Nov 16, 2007·Nature Reviews. Cancer·Alfredo Fusco, Monica Fedele
Apr 17, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kazuo MotoyamaMasaki Mori
May 3, 2008·International Journal of Cancer. Journal International Du Cancer·Anastasia MalekOleg Tchernitsa
May 29, 2008·Molecular Cancer Research : MCR·Francescopaolo Di CelloLinda M S Resar
Sep 17, 2008·Oncogene·T L Yuan, L C Cantley
Nov 28, 2012·Developmental Cell·Zhizhong LiDavid J Glass
May 2, 2013·The Journal of Investigative Dermatology·Leon RaskinStephen B Gruber

❮ Previous
Next ❯

Citations

Dec 31, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yang LiChengyuan Ma
Jul 29, 2017·Thoracic Cancer·Xiaotian GaoZeqing Song
Jun 27, 2019·Journal of Cellular Physiology·Behzad MansooriBehzad Baradaran
Jun 8, 2019·International Journal of Molecular Medicine·Shuo YangMingfeng Zhao
Jun 27, 2020·Technology in Cancer Research & Treatment·Ya-Dong WangMao-Qi Xu
Aug 23, 2019·International Journal of Oncology·Shizhen ZhangXiaochen Wang
May 6, 2020·International Journal of Molecular Sciences·Uchenna UnachukwuJeanine D'Armiento
Jun 7, 2020·Cancers·Angela MinerviniFrancesco Albano
Oct 18, 2020·Translational Oncology·Xin WangJingjing Wu
Mar 7, 2021·Genes·Behzad MansooriBehzad Baradaran
Sep 14, 2021·Frontiers in Molecular Biosciences·Zhouying WuLan Yu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Acute Myeloid Leukemia

Acute myeloid leukemia (AML) is a clinically and genetically heterogeneous disease with approximately 20,000 cases per year in the United States. AML also accounts for 15-20% of all childhood acute leukemias, while it is responsible for more than half of the leukemic deaths in these patients. Here is the latest research on this disease.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

AML: Role of LSD1 by CRISPR (Keystone)

Find the latest rersearrch on the ability of CRISPR-Cas9 mutagenesis to profile the interactions between lysine-specific histone demethylase 1 (LSD1) and chemical inhibitors in the context of acute myeloid leukemia (AML) here.