Amplified oxygen reduction signal at a Pt-Sn-modified TiO2 nanocomposite on an electrochemical aptasensor

Biosensors & Bioelectronics
Lele LiDanny K Y Wong

Abstract

In this work, a metallic composite with strong electrocatalytic property was designed by uniformly decorating Pt and Sn nanoparticles on the surface of TiO2 nanorods (Pt-Sn@TiO2). A detection scheme was then developed based on a dual signal amplification strategy involving the Pt-Sn@TiO2 composite and exonuclease assisted target recycling. The Pt-Sn@TiO2 composite exhibited an enhanced oxygen reduction current owing to the synergistic effect between Pt and Sn, as well as high exposure of Pt (111) crystal face. Initially, a Pt-Sn@TiO2 modified glassy carbon electrode produced an amplified electrochemical signal for the reduction of dissolved oxygen in the analyte solution. Next, a DNA with a complementary sequence to a streptomycin aptamer (cDNA) was immobilised on the Pt-Sn@TiO2 modified electrode, followed by the streptomycin aptamer that hybridised with cDNA. The corresponding oxygen reduction current was diminished by 51% attributable to the hindrance from the biomolecules. After a mixture of streptomycin and RecJf exonuclease was introduced, both the streptomycin-aptamer complex and the cDNA were cleaved from the electrode, making the Pt-Sn and Pt (111) surface available for oxygen reduction. RecJf would also release strept...Continue Reading

Citations

Dec 10, 2020·Science China. Chemistry·Yongxi ZhaoChunhai Fan
Jun 27, 2021·Analytica Chimica Acta·Lucas Vinícius de FariaRodrigo Alejandro Abarza Munoz
Oct 12, 2021·Critical Reviews in Biotechnology·Chitra Padmakumari KurupMinhaz Uddin Ahmed

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