Amplified RLR signaling activation through an interferon-stimulated gene-endoplasmic reticulum stress-mitochondrial calcium uniporter protein loop

Scientific Reports
Jinbo ChengZengqiang Yuan

Abstract

Type I interferon (IFN-I) is critical for a host against viral and bacterial infections via induction of hundreds of interferon-stimulated genes (ISGs), but the mechanism underlying the regulation of IFN-I remains largely unknown. In this study, we first demonstrate that ISG expression is required for optimal IFN-β levels, an effect that is further enhanced by endoplasmic reticulum (ER) stress. Furthermore, we identify mitochondrial calcium uniporter protein (MCU) as a mitochondrial antiviral signaling protein (MAVS)-interacting protein that is important for ER stress induction and amplified MAVS signaling activation. In addition, by performing an ectopic expression assay to screen a library of 117 human ISGs for effects on IFN-β levels, we found that tumor necrosis factor receptor 1 (TNFR1) significantly increases IFN-β levels independent of ER stress. Altogether, our findings suggest that MCU and TNFR1 are involved in the regulation of RIG-I-like receptors (RLR) signaling.

References

Sep 2, 1999·Japanese Journal of Clinical Oncology·S Kato
Aug 30, 2005·Nature Immunology·Taro KawaiShizuo Akira
Sep 13, 2005·Molecular Cell·Liang-Guo XuHong-Bing Shu
Feb 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Michal Caspi TalAkiko Iwasaki
May 12, 2009·Ageing Research Reviews·Monika Puzianowska-Kuznicka, Jacek Kuznicki
Jun 17, 2009·Biochimica Et Biophysica Acta·György Csordás, György Hajnóczky
Sep 11, 2009·The Journal of Clinical Investigation·Jenelle M TimminsIra Tabas
Dec 19, 2009·EMBO Reports·Céline CastanierDamien Arnoult
Jan 16, 2010·Science·Jan Rehwinkel, Caetano Reis e Sousa
Mar 25, 2010·Science Signaling·Mark P Mattson
May 18, 2011·Transplantation·Maria Cecilia S FreitasJerzy W Kupiec-Weglinski
Feb 11, 2012·Current Opinion in Virology·David E LevyJoan E Durbin
Mar 13, 2013·Current Opinion in Cell Biology·Shuliang ChenSusan Ferro-Novick
Oct 15, 2013·Journal of Molecular Biology·Jana L Jacobs, Carolyn B Coyne
Jan 23, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Feng MaGenhong Cheng

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Citations

Feb 18, 2017·International Journal of Molecular Sciences·Yajin LiaoJinbo Cheng
Sep 29, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maolin GeJianguo Wu
Jan 31, 2019·International Journal of Molecular Sciences·Yuan DongLin Hou
Feb 1, 2017·Cell Death and Differentiation·Jinbo ChengZengqiang Yuan
Aug 8, 2019·Open Biology·Alexander J AndersonDiana Stojanovski
Mar 19, 2020·Journal of Clinical Medicine·Sonia MissiroliCarlotta Giorgi
Dec 29, 2016·Journal of Neuroscience Research·Xiangxi KongJinbo Cheng
Sep 22, 2019·Cells·Dmitry NamgaladzeBernhard Brüne
Oct 28, 2017·International Journal of Dermatology·Julie CastagnaAnne S Dupond
Apr 25, 2020·Frontiers in Physiology·Devasena Ponnalagu, Harpreet Singh
Jul 9, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anna Kicinska, Wieslawa Jarmuszkiewicz
Aug 26, 2018·Nature Reviews. Molecular Cell Biology·Carlotta GiorgiPaolo Pinton
Mar 30, 2021·Mitochondrion·Swagatika PandaGulam Hussain Syed
Nov 28, 2021·Pharmaceuticals·Gabsik YangJoo Young Lee

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Methods Mentioned

BETA
nuclear translocation
tandem affinity purification
transfection
PCR

Software Mentioned

Graphpad

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