Amplified telomerase analysis by using rotating magnetic particles: the rapid and sensitive detection of cancer cells

Chembiochem : a European Journal of Chemical Biology
Yossi WeizmannItamar Willner

Abstract

A highly sensitive telomerase detection method that involves amplified telomerase analysis and the use of rotating magnetic particles has been developed. Magnetic particles, functionalized with a primer (1) that is recognized by telomerase, are mixed with a nucleotide mixture that includes biotinylated-dUTP, and telomerase-induced elongation of the primers proceeds with simultaneous biotin incorporation. Avidin-Horseradish peroxidase conjugate, coupled to biotin labels, yields the biocatalytic functional particles. Mixing the resulting particles with naphthoquinone-modified magnetic particles enables the optoelectronic detection of telomerase. Attraction of the magnetic particles to an electrode, followed by rotation of the particles, causes the electrocatalytic reduction of O(2) to H(2)O(2) and HRP-catalyzed oxidation of luminol (3); this results in chemilumunescence. The intensity of the emitted light depends on the telomerase content of the sample and the rotation speed of the particles. A minimum number of 10 cancer cells could be detected.

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Citations

Nov 13, 2004·Angewandte Chemie·Eugenii Katz, Itamar Willner
Dec 11, 2007·Journal of Biomedical Materials Research. Part a·Min SongDegang Fu
Nov 30, 2011·Chembiochem : a European Journal of Chemical Biology·Xiaolei ZuoKevin W Plaxco
Apr 18, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Jirí Fajkus
Sep 10, 2014·Biosensors & Bioelectronics·Yan LiNengqin Jia

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