Amplitude Modulation Mode of Scanning Ion Conductance Microscopy

Journal of Laboratory Automation
Peng LiGuangyong Li

Abstract

Live-cell imaging at the nanoscale resolution is a hot research topic in the field of life sciences for the direct observation of cellular biological activity. Scanning ion conductance microscopy (SICM) is one of the few effective imaging tools for live-cell imaging at the nanoscale resolution. However, there are various problems in existing scanning modes. The hopping and AC modes suffer from low speed, whereas the DC mode is prone to instability because of the DC drift and external electrical interference. In this article, we propose an amplitude modulation (AM) mode of SICM, which employs an AC voltage to enhance the stability and improve the scanning speed. In this AM mode, we introduce a capacitance compensation method to eliminate capacitance effect and use the amplitude of the AC current component to control the tip movement. Experimental results on polydimethylsiloxane samples verify the validity of the AM mode and demonstrate an improved performance of both speed and stability of this new mode.

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Citations

Jul 22, 2015·Journal of Laboratory Automation·Xianting Ding
May 10, 2017·Proceedings. Mathematical, Physical, and Engineering Sciences·Ashley PagePatrick R Unwin
Jan 3, 2020·The Review of Scientific Instruments·Shinji WatanabeToshio Ando
Apr 9, 2020·The Review of Scientific Instruments·Jian ZhuangZhiwu Wang
Oct 11, 2017·Analytical Chemistry·David PerryPatrick R Unwin
Dec 10, 2020·Chemical Reviews·Cheng ZhuLane A Baker

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Methods Mentioned

BETA
optical microscopy
AFM

Software Mentioned

Labview
Linux
SICM

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