AmpliVue Is a Practical and Timely Test for the Detection of HSV From Keratitis Specimens

Eye & Contact Lens
R P KowalskiAlex Mammen

Abstract

The laboratory diagnostic detection of herpes simplex virus (HSV) from eye samples must be practical, timely, and definitive for appropriate therapy. Although polymerase chain reaction (PCR) and/or cell culture can be definitive, HSV results can be delayed. Enzyme Linked Virus Inducible System (ELVIS) is a test that can provide results within 24 to 48 hr. We evaluated "AmpliVue HSV 1+2 Assay" as a molecular colorimetric test that can detect HSV (1 or 2) DNA within 1 hr. Cornea/conjunctival samples were tested retrospectively with AmpliVue against 53 true-positive and 20 true-negative specimens collected in chlamydial transport medium. All clinical specimens were tested by cell culture isolation, PCR, and ELVIS for routine patient care. The sensitivity of AmpliVue against ocular samples that were both culture-positive and PCR-positive was 84%. The specificity of AmpliVue was 100%. Only one clinical sample was HSV-2 positive, whereas all others tested positive for HSV-1. Based on PCR-positive and cell culture-negative samples, AmpliVue (11 of 17) tested more positive than ELVIS (0 of 17) (P=0.003, Fisher Exact). AmpliVue is moderately sensitive and highly specific as a practical and timely diagnostic test for detecting ocular HSV...Continue Reading

References

Aug 15, 1991·American Journal of Ophthalmology·R P KowalskiR C Arffa
Feb 3, 2000·Journal of Clinical Microbiology·M J EspyT F Smith
Jul 6, 2002·Archives of Ophthalmology·Regis P KowalskiJerold S Gordon
Jan 30, 2004·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Julia SchmutzhardLena Grillner
Aug 26, 2015·Diagnostic Microbiology and Infectious Disease·Jeanette W P TeoRaymond T P Lin

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Citations

Mar 11, 2021·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Sana ArshadNicole A Carnt
Nov 14, 2020·Survey of Ophthalmology·Stephanie Hiu Ling PoonKendrick Co Shih
Jun 3, 2021·Clinical Microbiology Reviews·Sixto M LealPeter H Gilligan
Jul 3, 2021·Infectious Disease Reports·Peuli NathAniruddha Ray

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