Amygdalin delays cell cycle progression and blocks growth of prostate cancer cells in vitro

Life Sciences
Jasmina MakarevićRoman A Blaheta

Abstract

Despite impressive survival benefits from new agents to treat metastasized prostate cancer (PCa), progressive drug resistance hinders long-term response and restricts the efficacy of subsequent therapy. Due to reported antitumor activity of amygdalin and growing popularity for complementary and alternative medicine the potential of this natural, widely used substance to exert antineoplastic effects on prostate cancer cells has been assessed. LNCaP (castration-sensitive), DU-145 and PC3 cells (castration-resistant) were exposed to different concentrations of amygdalin for 24h or 2weeks. Cell growth was measured by the MTT test, clonal formation by the clonogenic assay. Flow cytometry served to investigate apoptosis and cell cycle phases. Cell cycle regulating proteins and the mTOR-akt signaling axis were analyzed by western blotting. Amygdalin dose-dependently diminished tumor cell growth with maximum effects at 10mg/ml. Apoptosis of PC3 and LNCaP but not of DU-145 cells was reduced, whereas colony formation was suppressed in all cell lines. A decrease in the number of G2/M- and S-phase cells along with an elevated number of G0/G1-phase cells was recorded. The cell cycle proteins cdk 1, cdk 2 and cdk 4 as well as cyclin A, cycli...Continue Reading

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Citations

Jan 6, 2018·International Journal of Molecular Sciences·Inamul HaqueSushanta K Banerjee
Jan 10, 2020·Environmental Science and Pollution Research International·Ehab ToussonThulfiqar Fawwaz Mutar
Mar 17, 2020·Journal of Biomolecular Structure & Dynamics·Khattab Al-Khafaji, Tugba Taskin Tok
Jul 13, 2019·Nutrition and Cancer·Jens ManiRoman A Blaheta
Jul 9, 2020·International Journal of Nanomedicine·Jie ZhouWenxi Gao
Feb 23, 2020·Seminars in Cancer Biology·Sajad FakhriAnupam Bishayee
Mar 3, 2020·Journal of Ethnopharmacology·Xiao-Yan HeFei Wang

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