Amylase inhibitors of pigeonpea (Cajanus cajan) seeds

Phytochemistry
Ashok P Giri, Manvendra S Kachole

Abstract

Pigeonpea (Cajanus cajan L) seeds were analysed quantitatively for amylase inhibitor (AI) activity and qualitatively, by an in-gel-detection method on polyacrylamide gels. At least four AI isoforms were identified in pigeonpea seeds. The AIs inhibit human salivary and bovine pancreatic amylase but fail to inhibit bacterial, fungal and endogenous amylase. Pigeonpea AIs were found to be active over a pH range of 4.5 to 9.5 and were heat labile. The isoelectric point of a major inhibitor is 6.2 AIs were tolerant to proteolysis by trypsin, chymotrypsin, bromelain and endogenous pigeonpea proteases. Pigeonpea AIs were synthesized during late seed development and also degraded during late germination. Addition of AIs or protease inhibitors (PIs) alone to a diet of Helicoverpa armigera larvae did not increase mortality. However, the larvae reared on a diet containing AIs and PIs in combination, showed increased mortality and adverse effects on larval growth and development. In vitro inhibition of Helicoverpa gut amylase revealed that only 22% activity is sensitive to inhibitors. Further investigations on interactions of pigeonpea AIs and PIs with Helicoverpa gut enzymes is necessary to develop strategies to strengthen defense mechanis...Continue Reading

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Citations

Feb 26, 2013·Journal of Agricultural and Food Chemistry·Fanrong ZengQiannan Li
Feb 22, 2002·European Journal of Biochemistry·Octávio L FrancoMaria F Grossi-De-Sá
Apr 27, 2010·Journal of Insect Physiology·Vinod D PardeManvendra S Kachole
May 20, 2009·Journal of Insect Physiology·Hemlata M KotkarAshok P Giri
Dec 5, 2014·Food Science & Nutrition·Katherine P MaloneyJonathan C Allen
Sep 23, 2014·Pest Management Science·Rimaljeet KaurGaurav K Taggar
Aug 27, 2005·Journal of Enzyme Inhibition and Medicinal Chemistry·Ludmila ZajoncovaPavel Pec
Jun 11, 2014·Critical Reviews in Food Science and Nutrition·Ming MiaoZhengyu Jin
May 8, 2009·Bioscience, Biotechnology, and Biochemistry·Xiaoyan HaoHao Ma
Mar 1, 2010·Journal of Dietary Supplements·Catherine UlbrichtWendy Weissner
Feb 16, 2021·Journal of Biomolecular Structure & Dynamics·Ruth M OgboyeKolade O Faloye
Aug 1, 2020·International Journal of Biological Macromolecules·Ashwini S RaneAshok P Giri
Aug 18, 2020·Biochimica Et Biophysica Acta. General Subjects·Ashwini S RaneAshok P Giri
Mar 7, 2008·Journal of Agricultural and Food Chemistry·Arnubio Valencia-JiménezMaria Fátima Grossi-De-Sá

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