Amylase measurement with 2-chloro-4-nitrophenyl maltotrioside as substrate

Clinica Chimica Acta; International Journal of Clinical Chemistry
A Y Foo, R Bais

Abstract

The use of 2-chloro-4-nitrophenyl maltotrioside (CNP-G3) as substrate to measure amylase (EC 3.2.1.1) activity in serum directly without the use of auxiliary enzymes was evaluated at two centres. The method was precise (within-run C.V. < 2% and between-run C.V. < 3%), there was no lag phase, background absorbance was low and there were minimal effects of pH changes. When compared with a method which uses 4,6-ethylidene (G7)-p-nitrophenyl (G1)-alpha-D-maltoheptaoside (EPS-G7) as substrate, the CNP-G3 method had greater sensitivity and longer reagent stability (21 days compared with 2 days at 4 degrees C). The activity measured with the CNP-G3 method correlated well with methods using either EPS-G7 and maltotetraose as substrates.

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Citations

Oct 7, 2009·Journal of Biosciences·Jay Kant Yadav, V Prakash
Jun 26, 2002·Letters in Applied Microbiology·C E Deutch
Dec 15, 2015·Biosensors & Bioelectronics·Satarupa DuttaDipankar Bandyopadhyay
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May 24, 2014·The Analyst·Juan ZhangGenxi Li
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Nov 30, 2013·Journal of Breath Research·M ZweifelA Boehler
Oct 16, 2019·Journal of Food Science·Kenji KuriyaHayato Umekawa
Nov 27, 2019·Doklady. Biochemistry and Biophysics·N M MalyginaA M Ivanov
Apr 23, 2020·Antonie van Leeuwenhoek·Charles E Deutch, Shanshan Yang
Jul 7, 2020·EFSA Journal·UNKNOWN EFSA Panel on Nutrition, Novel Foods and Food Allergens (EFSA NDA Panel)Alfonso Siani
Jul 26, 2000·Biochemical Engineering Journal·D HeS Yao
Oct 12, 2010·Annals of Clinical Biochemistry·Simon J F Harper, Sarah Cheslyn-Curtis

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