Amylase-Protected Ag Nanodots for in vivo Fluorescence Imaging and Photodynamic Therapy of Tumors

International Journal of Nanomedicine
Shuguang WenPengcheng He

Abstract

Fluorescent metallic nanodots (NDs) have become a promising nanoprobe for a wide range of biomedical applications. Because Ag NDs have a high tendency to be oxidized, their synthesis and storage are a big challenge. Thus, the method for preparing stable Ag NDs is urgently needed. Surface modification and functionalization can enrich the capability of Ag NDs. In this work, fluorescent Ag NDs were synthesized in deoxygenated water by using porcine pancreatic α-amylase (PPA) as the stabilizing/capping agent. The absorption and fluorescence of PPA-protected Ag NDs (PPA@AgNDs) were measured with a spectrophotometer and a spectrofluorometer, respectively. The morphology of PPA@AgNDs was characterized by high-angle annular dark-field (HAADF) scanning transmission electron microscopy (STEM). The biocompatibility of PPA@AgNDs was evaluated by tetrazolium (MTT)-based assay. PolyLys-Cys-SH (sequence: KKKKKKC) peptides were conjugated to PPA@AgNDs via heterobifunctional crosslinkers. PolyLys-Cys-linked PPA@AgNDs absorbed 5-aminolevulinic acid (ALA) by electrostatic interaction at physiological pH. The capability of tumor targeting was evaluated by intravenously injecting PPA@AgND-ALA into 4T1 breast cancer xenograft mouse models. Photodyna...Continue Reading

References

Aug 25, 2004·Physical Review Letters·Jie ZhengRobert M Dickson
Jun 30, 2005·Journal of the American Chemical Society·Hironori TsunoyamaTatsuya Tsukuda
Jan 7, 2009·Journal of the American Chemical Society·Jianping XieJackie Y Ying
Jun 17, 2010·Nano Letters·Zhikun Wu, Rongchao Jin
Jul 21, 2010·Nanoscale·Rongchao Jin
Sep 15, 2010·Nano Letters·Beatriz Santiago GonzálezJosé M Gaspar Martinho
Mar 17, 2011·Nanoscale·Isabel Díez, Robin H A Ras
Nov 15, 2011·Chemical Society Reviews·Sungmoon ChoiJunhua Yu
Feb 19, 2013·Advanced Materials·R SachanR Kalyanaraman
Mar 29, 2013·Accounts of Chemical Research·Gao Li, Rongchao Jin
Jun 8, 2013·The Journal of Physical Chemistry Letters·Jeffrey T PettyRobert M Dickson
Mar 5, 2014·Accounts of Chemical Research·Stefan Knoppe, Thomas Bürgi
Jan 20, 2011·The Journal of Physical Chemistry Letters·Christine M Aikens
Feb 23, 2017·Advances in Colloid and Interface Science·Youkun ZhengXuemei Wang
Feb 25, 2017·Physical Chemistry Chemical Physics : PCCP·B A RussellY Chen

❮ Previous
Next ❯

Methods Mentioned

BETA
transmission electron microscopy
fluorescence-activated cell sorting
surface plasma resonance
fluorescence imaging
dynamic light scattering
FACS
xenograft

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved