Amylin proprotein processing generates progressively more amyloidogenic peptides that initially sample the helical state.

Biochemistry
Isaac T YonemotoJeffery W Kelly

Abstract

Human amylin, or islet amyloid polypeptide, is a peptide cosecreted with insulin by the beta cells of the pancreatic islets of Langerhans. The 37-residue, C-terminally amidated human amylin peptide derives from a proprotein that undergoes disulfide bond formation in the endoplasmic reticulum and is then subjected to four enzymatic processing events in the immature secretory granule. Human amylin forms both intracellular and extracellular amyloid deposits in the pancreas of most type II diabetic subjects, likely reflecting compromised secretory cell function. In addition, amylin processing intermediates, postulated to initiate intracellular amyloidogenesis, have been reported as components of intracellular amyloid in beta cells. We investigated the amyloidogenicity of amylin and its processing intermediates in vitro. Chaotrope-denatured amylin and amylin processing intermediates were subjected to size exclusion chromatography, affording high concentrations of monomeric peptides. NMR studies reveal that human amylin samples helical conformations. Under conditions mimicking the immature secretory granule (37 degrees C, pH 6), amylin forms amyloid aggregates more rapidly than its processing intermediates, and more rapidly than its ...Continue Reading

References

Sep 1, 1991·Peptides·A BalasubramaniamW T Chance
Jun 15, 1990·Biochemical and Biophysical Research Communications·K JordanK H Johnson
May 1, 1989·Diabetologia·J C Hutton
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P WestermarkK H Johnson
Nov 14, 1986·Biochemical and Biophysical Research Communications·P WestermarkK Sletten
Sep 1, 1994·The Journal of Cell Biology·L OrciR G Anderson
Aug 30, 1994·Proceedings of the National Academy of Sciences of the United States of America·E J de KoningJ S Verbeek
Jul 30, 1997·Biochemical and Biophysical Research Communications·S JanciauskieneB Ahrén
Mar 10, 1998·European Journal of Biochemistry·A KapurniotuR Bucala
Apr 7, 1999·Journal of Molecular Biology·R KayedA Kapurniotu
Nov 30, 2000·Chemistry & Biology·M KrampertA Kapurniotu
Dec 4, 2001·Biochimica Et Biophysica Acta·E T Jaikaran, A Clark
Feb 19, 2003·Journal of Molecular Biology·Janelle GreenUeli Aebi
Apr 3, 2003·Experimental Gerontology·Lucy MarzbanC Bruce Verchere
Dec 9, 2003·Journal of Molecular Biology·Jennifer L Larson, Andrew D Miranker
Jan 6, 2004·The Journal of Biological Chemistry·Janelle D GreenUeli Aebi
Oct 16, 2004·Science·Umut OzcanGökhan S Hotamisligil
Dec 4, 2004·Protein Science : a Publication of the Protein Society·Bon W Koo, Andrew D Miranker
Dec 8, 2005·Biochemistry·Andisheh Abedini, Daniel P Raleigh
Nov 25, 2006·Protein Science : a Publication of the Protein Society·Jessica A Williamson, Andrew D Miranker
Feb 16, 2008·Science·William E BalchJeffery W Kelly
Mar 4, 2008·Endocrine Reviews·Leena HaatajaPeter C Butler

❮ Previous
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Citations

Feb 22, 2012·Applied Biochemistry and Biotechnology·Wojciech PulawskiSlawomir Filipek
Sep 29, 2011·Accounts of Chemical Research·Jeffrey R BrenderAyyalusamy Ramamoorthy
Nov 8, 2008·Biochemistry·Ravi Prakash Reddy NangaAyyalusamy Ramamoorthy
Dec 9, 2010·Journal of the American Chemical Society·Gai LiuNoel D Lazo
Sep 16, 2011·Journal of the American Chemical Society·Lu WangJames L Skinner
May 22, 2009·Journal of the American Chemical Society·Ravi Prakash Reddy NangaAyyalusamy Ramamoorthy
Dec 3, 2009·Journal of the American Chemical Society·Nicholas F DupuisMichael T Bowers
Nov 3, 2012·The Journal of Physical Chemistry. B·Janarthanan KrishnamoorthyAyyalusamy Ramamoorthy
Jun 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Pik K ChanJoan Selverstone Valentine
Jul 15, 2009·Protein Engineering, Design & Selection : PEDS·John R CortNiels H Andersen
Jul 15, 2009·Protein Engineering, Design & Selection : PEDS·Andisheh Abedini, Daniel P Raleigh
Jul 3, 2013·AAPS PharmSciTech·Luiz Henrique GuerreiroLuís Maurício T R Lima
Jan 1, 2014·Biochemistry·Suman JhaAndrei T Alexandrescu
Apr 23, 2013·BioMed Research International·Karen Pillay, Patrick Govender
Apr 26, 2013·The Journal of Chemical Physics·Sadanand SinghJuan J de Pablo
Apr 23, 2014·Protein Expression and Purification·Iga KosickaNiels Chr Nielsen
Feb 14, 2012·Colloids and Surfaces. B, Biointerfaces·Luiz Henrique GuerreiroLuís Maurício T R Lima
Apr 7, 2015·ACS Chemical Biology·Dahabada H J LopesGal Bitan
Nov 3, 2015·Canadian Journal of Physiology and Pharmacology·Jessica S Fortin, Marie-Odile Benoit-Biancamano
May 28, 2011·Journal of Molecular Biology·Samer SalamekhAyyalusamy Ramamoorthy
Aug 22, 2014·Journal of the American Chemical Society·Anna C SusaMichael T Bowers
Aug 4, 2009·Journal of Molecular Biology·Jessica A WilliamsonAndrew D Miranker
Jun 9, 2009·Chemistry and Physics of Lipids·Maarten F M Engel
Jul 22, 2009·Protein Science : a Publication of the Protein Society·Isaac T YonemotoJeffery W Kelly
May 29, 2009·Protein Science : a Publication of the Protein Society·Jed J W WiltziusDavid Eisenberg
Jul 4, 2009·Chembiochem : a European Journal of Chemical Biology·Rajesh MishraRoland Winter
May 4, 2011·Chembiochem : a European Journal of Chemical Biology·Taku YoshiyaYoshiaki Kiso
Jun 14, 2013·Proteins·Yang LiKun Huang
Jan 14, 2010·Biopolymers·Karen MuthusamyThavendran Govender
Dec 2, 2009·Biophysical Journal·Sara M VaianaJames Hofrichter
Dec 10, 2015·Journal of Diabetes Research·Zhenyu QianGuanghong Wei
Dec 10, 2015·Journal of Diabetes Research·Amit PithadiaAyyalusamy Ramamoorthy

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