Amyloid beta-peptide decreases neuronal glucose uptake despite causing increase in GLUT3 mRNA transcription and GLUT3 translocation to the plasma membrane

Experimental Neurology
T PrapongEtsuro Uemura

Abstract

Amyloid beta-peptide (Abeta) has been shown to impair glucose uptake in cultured hippocampal neurons and shortens their survival time. Abeta appears to inhibit neuronal glucose uptake by activating Gs-coupled receptors and the cAMP-PKA system. In this study, Abeta inhibition of neuronal glucose uptake was studied by assaying translocation of glucose transporter isoform GLUT3, transcription of GLUT3 mRNA, and fusion of GLUT3-containing vesicles with the plasma membrane. Cultured hippocampal neurons exposed to 10 microM Abeta25-35 or Abeta1-40 for 3 or 24 h showed a significant decrease in glucose uptake. To assess the regulatory role of Abeta on neuronal glucose uptake, translocation of GLUT3 from the cytosol to the plasma membrane was studied by the plasma membrane lawn assay and transcription of GLUT3 mRNA by in situ hybridization. In spite of a decrease in glucose uptake, Abeta25-35 and Abeta1-40 (10 microM) markedly promoted GLUT3 translocation to the plasma membrane by 30 min. Abeta25-35 also up-regulated transcription of GLUT3 mRNA by 12 h. High extracellular K(+) increased immunolabeling of the exofacial (i.e., extracellular) epitope of GLUT3 at the plasma membrane and Abeta25-35 inhibited this increase. Based on these da...Continue Reading

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Citations

Jul 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Fei LiuCheng-Xin Gong
Dec 24, 2013·Cellular and Molecular Life Sciences : CMLS·Lawrence R GrayEric B Taylor
Feb 25, 2015·Frontiers in Neuroscience·Igor AllamanPierre J Magistretti
Mar 3, 2004·Journal of Neuroscience Research·Bo-Chul ShinSherin U Devaskar
Apr 22, 2003·Journal of Neuroscience Research·Jigisha R Patel, Gregory J Brewer
Jan 17, 2009·Reviews in the Neurosciences·Jianmin Chen, Karl Herrup
Dec 18, 2012·Journal of Neurochemistry·Misha ZilberterYuri Zilberter
May 4, 2017·Journal of Neuroscience Research·Yuri Zilberter, Misha Zilberter
Jun 7, 2019·World Journal of Stem Cells·Kate Elizabeth Hawkins, Michael Duchen
Aug 24, 2021·Metabolism: Clinical and Experimental·Wuxue PengLifen Chen

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