Amyloid-beta peptide degradation in cell cultures by mycoplasma contaminants.

BMC Research Notes
Haitian ZhaoPhilippe Marambaud

Abstract

Cell cultures have become an indispensable tool in Alzheimer's disease research for studying amyloid-beta (Abeta) metabolism. It is estimated that up to 35% of cell cultures in current use are infected with various mycoplasma species. In contrast with common bacterial and fungal infections, contaminations of cell cultures with mycoplasmas represent a challenging issue in terms of detectability and prevention. Mycoplasmas are the smallest and simplest self-replicating bacteria and the consequences of an infection for the host cells are variable, ranging from no apparent effect to induction of apoptosis. Here we present evidence that mycoplasmas from a cell culture contamination are able to efficiently and rapidly degrade extracellular Abeta. As a result, we observed no accumulation of Abeta in the conditioned medium of mycoplasma-positive cells stably transfected with the amyloid-beta precursor protein (APP). Importantly, eradication of the mycoplasma contaminant - identified as M. hyorhinis - by treatments with a quinolone-based antibiotic, restored extracellular Abeta accumulation in the APP-transfected cells. These data show that mycoplasmas degrade Abeta and thus may represent a significant source of variability when compari...Continue Reading

References

Jun 8, 1989·Nature·R J HayT R Chen
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·S TsaiS C Lo
Mar 29, 2001·Physiological Reviews·D J Selkoe
Apr 4, 2002·In Vitro Cellular & Developmental Biology. Animal·Cord C Uphoff, Hans G Drexler
Apr 4, 2002·In Vitro Cellular & Developmental Biology. Animal·Cord C Uphoff, Hans G Drexler
Jun 19, 2002·Proceedings of the National Academy of Sciences of the United States of America·Xuefeng XiaHui Zheng
Feb 1, 2005·Annals of the New York Academy of Sciences·Anthony J TurnerNatalia N Nalivaeva
Sep 16, 2005·The Journal of Biological Chemistry·Philippe MarambaudPeter Davies

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Citations

Apr 11, 2009·Microbial Cell Factories·Jacques M MathieuPedro Jj Alvarez
Apr 12, 2013·Journal of Nanobiotechnology·M Charl MoolmanNynke H Dekker
Mar 13, 2014·Revue neurologique·N B BolshetteR Gogoi
Nov 15, 2011·Biologicals : Journal of the International Association of Biological Standardization·Elisabetta MariottiLuigi Del Vecchio
Jan 5, 2011·Letters in Applied Microbiology·M EterpiV Thomas
Apr 15, 2016·Analytical and Bioanalytical Chemistry·Tao YuHuichang Bi
Jan 19, 2010·The Journal of Biological Chemistry·Valérie VingtdeuxPhilippe Marambaud
Sep 21, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Valérie VingtdeuxPhilippe Marambaud
Jun 8, 2017·The Journal of Clinical Endocrinology and Metabolism·Gerard BlascoJosé-Manuel Fernandez-Real
Jul 10, 2021·EcoSal Plus·Gwanggyu SunMarkus W Covert

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Methods Mentioned

BETA
light microscopy
PCR
restriction digests

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