Amyloid-beta peptide induces temporal membrane biphasic changes in astrocytes through cytosolic phospholipase A2.

Biochimica Et Biophysica Acta
Jacob B HicksJames C-M Lee

Abstract

Oligomeric amyloid-beta peptide (Abeta) is known to induce cytotoxic effects and to damage cell functions in Alzheimer's disease. However, mechanisms underlying the effects of Abeta on cell membranes have yet to be fully elucidated. In this study, Abeta 1-42 (Abeta(42)) was shown to cause a temporal biphasic change in membranes of astrocytic DITNC cells using fluorescence microscopy of Laurdan. Abeta(42) made astrocyte membranes became more molecularly-disordered within the first 30 min to 1 h, but gradually changed to more molecularly-ordered after 3 h. However, Abeta(42) caused artificial membranes of vesicles made of rat whole brain lipid extract to become more disordered only. The trend for more molecularly-ordered membranes in astrocytes induced by Abeta(42) was abrogated by either an NADPH oxidase inhibitor, apocynin, or an inhibitor of cytosolic phospholipase A(2) (cPLA(2)), but not by an inhibitor of calcium-independent PLA(2) (iPLA(2)). Apocynin also suppressed the increased production of superoxide anions (O(2)(-)) and phosphorylation of cPLA(2) induced by Abeta(42). In addition, hydrolyzed products of cPLA(2), arachidonic acid (AA), but not lysophosphatidylcholine (LPC) caused astrocyte membranes to become more molec...Continue Reading

References

Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E H RadanyC F Deschepper
Oct 1, 1992·Experimental Cell Research·T ParasassiE Gratton
Jun 1, 1990·Biophysical Journal·T ParasassiE Gratton
Jul 1, 1994·American Journal of Respiratory Cell and Molecular Biology·J StolkA J Verhoeven
May 16, 1994·Biochemical and Biophysical Research Communications·H HartmannW E Müller
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·N ArispeH B Pollard
Apr 18, 1998·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·W E MüllerW F Gattaz
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·M P LambertW L Klein
May 20, 1999·Annual Review of Pharmacology and Toxicology·J BalsindeE A Dennis
Feb 27, 2001·Biophysical Journal·C DietrichE Gratton
Mar 20, 2001·Biotechnology and Bioengineering·J C LeeD E Discher
Apr 11, 2001·The Journal of Biological Chemistry·F M HarrisJ D Bell
Jul 5, 2001·The Journal of Biological Chemistry·S A WaschukJ A McLaurin
Aug 7, 2001·Journal of Neuropathology and Experimental Neurology·A NunomuraM A Smith
Aug 24, 2001·Journal of Molecular Biology·C M YipJ McLaurin
Mar 2, 2002·Journal of Biochemistry·Makoto Murakami, Ichiro Kudo
Jun 11, 2002·Journal of Molecular Biology·Christopher M YipJoAnne McLaurin
Jun 12, 2002·The Journal of Biological Chemistry·Karie N DahlgrenMary Jo LaDu
Oct 26, 2002·Science·Dennis J Selkoe
Mar 22, 2003·Biochimica Et Biophysica Acta·W Gibson WoodWalter E Müller
Dec 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Katharina GausWendy Jessup
Jan 16, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Andrey Y AbramovMichael R Duchen
Feb 22, 2005·The Journal of Biological Chemistry·Angelo DemuroCharles G Glabe
Apr 15, 2005·Journal of Neural Transmission·E L SchaefferW F Gattaz
Jun 21, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kenneth E ChapmanChristopher M Waters

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Citations

May 4, 2010·Molecular Neurobiology·Xiaoguang YangJames C-M Lee
Mar 25, 2009·Antioxidants & Redox Signaling·Silvia Sorce, Karl-Heinz Krause
Jul 30, 2010·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Noriyuki ShibataMakio Kobayashi
Jun 12, 2012·Oxidative Medicine and Cellular Longevity·Sonia Gandhi, Andrey Y Abramov
Feb 21, 2014·Molecular Neurobiology·Xiaoguang YangJames C-M Lee
Jun 16, 2015·Frontiers in Neuroscience·Donghui ZhuSholpan Askarova
Jan 11, 2011·Journal of Neurochemistry·James C-M LeeGrace Y Sun
Mar 5, 2019·Metabolomics : Official Journal of the Metabolomic Society·Mingyong ZhangYifeng Chai
Jul 17, 2020·Computational and Structural Biotechnology Journal·Yue LiuNady Braidy
Apr 11, 2021·Biogerontology·I AlmeidaA Nunes

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