PMID: 9450547Feb 5, 1998Paper

Amyloid beta-protein (A beta) associated with lipid molecules: immunoreactivity distinct from that of soluble A beta

FEBS Letters
K YanagisawaY Ihara

Abstract

We previously identified a novel amyloid beta-protein (A beta), that binds to GM1 ganglioside, in brains exhibiting the early pathological changes of AD. In this study, we raised monoclonal antibodies, using membrane fractions containing abundant GM1 ganglioside-bound A beta as antigens. Monoclonal antibody 4396, produced in this study, immunoprecipitates A beta42 in the membrane fractions of brains with diffuse plaques, but does not react with soluble A beta42 or GM1 ganglioside. Furthermore, this antibody recognizes the A beta bound to lipid vesicles containing GM1 ganglioside, and unexpectedly, phosphatidylinositol. In contrast, a control anti-A beta monoclonal antibody does not recognize the A beta bound to these lipid vesicles. These results indicate that A beta associated with lipids has an immunoreactivity distinct from that of soluble A.

References

Dec 1, 1990·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P GilbertW W Minuth
Jun 28, 1994·Biochemistry·J TalafousM G Zagorski
Nov 10, 1995·The Journal of Biological Chemistry·B L MartinB A Yankner
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A M WertkinV M Lee
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·P J TienariK Beyreuther
Apr 15, 1997·European Journal of Biochemistry·J McLaurin, A Chakrabartty
Jun 27, 1997·The Journal of Biological Chemistry·C Wild-BodeC Haass

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Citations

Mar 31, 2011·Nature Reviews. Neuroscience·Gilbert Di Paolo, Tae-Wan Kim
Mar 27, 2003·Nature Neuroscience·Luigi PuglielliDora M Kovacs
Jun 17, 2004·Journal of Neurochemistry·Naoki YamamotoKatsuhiko Yanagisawa
May 25, 2005·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Katsuhiko Yanagisawa
Nov 8, 2005·Journal of Neurochemistry·Naoki YamamotoKatsuhiko Yanagisawa
Apr 22, 1999·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·A PotempskaD L Miller
Aug 15, 2000·Journal of Structural Biology·D G Lynn, S C Meredith
Jul 19, 2000·Biochimica Et Biophysica Acta·A E RoherM R Emmerling
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Olena DobrovolskaØyvind Halskau
Nov 10, 2009·Progress in Lipid Research·R TakechiJ C L Mamo

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