Amyloid precursor-like protein 1 (APLP1) exhibits stronger zinc-dependent neuronal adhesion than amyloid precursor protein and APLP2

Journal of Neurochemistry
Magnus C MayerGerhard Multhaup

Abstract

The amyloid precursor protein (APP) and its paralogs, amyloid precursor-like protein 1 (APLP1) and APLP2, are metalloproteins with a putative role both in synaptogenesis and in maintaining synapse structure. Here, we studied the effect of zinc on membrane localization, adhesion, and secretase cleavage of APP, APLP1, and APLP2 in cell culture and rat neurons. For this, we employed live-cell microscopy techniques, a microcontact printing adhesion assay and ELISA for protein detection in cell culture supernatants. We report that zinc induces the multimerization of proteins of the amyloid precursor protein family and enriches them at cellular adhesion sites. Thus, zinc facilitates the formation of de novo APP and APLP1 containing adhesion complexes, whereas it does not have such influence on APLP2. Furthermore, zinc-binding prevented cleavage of APP and APLPs by extracellular secretases. In conclusion, the complexation of zinc modulates neuronal functions of APP and APLPs by (i) regulating formation of adhesion complexes, most prominently for APLP1, and (ii) by reducing the concentrations of neurotrophic soluble APP/APLP ectodomains. Earlier studies suggest a function of the amyloid precursor protein (APP) family proteins in neuron...Continue Reading

References

Jul 25, 1994·FEBS Letters·L HesseG Multhaup
Feb 17, 2000·Neurochemistry International·E J CoulsonC L Masters
Mar 29, 2001·Physiological Reviews·D J Selkoe
Dec 31, 2003·The Journal of Biological Chemistry·Qiming Li, Thomas C Südhof
Sep 30, 2005·The EMBO Journal·Peter SobaKonrad Beyreuther
Aug 1, 2006·Lancet·Kaj BlennowHenrik Zetterberg
Apr 5, 2007·Nature Protocols·Anne C von PhilipsbornFriedrich Bonhoeffer
Jun 30, 2007·Annual Review of Neuroscience·Lawrence ShapiroDavid R Colman
Dec 22, 2007·Biophysical Journal·Michelle A DigmanEnrico Gratton
Jan 30, 2008·Current Protocols in Cell Biology·M J Humphries
Nov 8, 2008·Journal of Visualized Experiments : JoVE·Lutz G W Hilgenberg, Martin A Smith
Jan 8, 2009·Journal of Cell Science·Daniela KadenGerd Multhaup
Sep 4, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Zilai WangHui Zheng
Oct 16, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Mako TakamiYasuo Ihara
May 24, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Christian HellriegelMoreno Zamai
Nov 11, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Stefano L SensiMichal Hershfinkel
Nov 16, 2011·Frontiers in Behavioral Neuroscience·Carlos Sindreu, Daniel R Storm
Jan 17, 2012·Journal of Molecular Biology·Sven O DahmsManuel E Than
Feb 23, 2012·Cold Spring Harbor Perspectives in Medicine·Ulrike C Müller, Hui Zheng
Jun 26, 2012·The EMBO Journal·Peer-Hendrik KuhnStefan F Lichtenthaler
Aug 14, 2012·Molecular and Cellular Neurosciences·Sheue-Houy TyanDara L Dickstein
Jan 25, 2013·Journal of Cell Science·S Ali M ShariatiAnnette Gärtner
May 15, 2013·Biophysical Journal·Kevin C CrosbyTheodorus W J Gadella
Nov 15, 2013·The Journal of Biological Chemistry·Fredrik OlssonJohan Lundkvist
May 24, 2014·The Journal of Biological Chemistry·Magnus C MayerGerhard Multhaup
Jul 8, 2014·Molecular and Cellular Neurosciences·Maja KlevanskiUlrike C Müller
Aug 15, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Frederik BaumkötterStefan Kins
Aug 21, 2014·Biophysical Journal·Malte HilschSalvatore Chiantia
Dec 19, 2014·Frontiers in Cellular Neuroscience·Ronny StahlStefan Kins

❮ Previous
Next ❯

Citations

Feb 16, 2017·Frontiers in Molecular Neuroscience·Klemens WildStefan Kins
Feb 1, 2018·Scientific Reports·Linda SchauenburgGerhard Multhaup
May 8, 2019·Journal of Neurochemistry·Alexander AugustStefan Kins
Nov 30, 2019·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Dimitri HefterMartin Korte
Oct 4, 2019·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Danielle K Bailey, Daniel J Kosman
Jul 15, 2018·Scientific Reports·Valentin DunsingSalvatore Chiantia
Dec 4, 2019·Frontiers in Immunology·Erik W MartinMyong-Hee Sung
Jun 24, 2020·Scientific Reports·Erwan LanchecChristine Lavoie
Jan 27, 2019·Scientific Reports·Hannah Sabeth SperberRoland Schwarzer
May 20, 2020·Progress in Neurobiology·Gael Barthet, Christophe Mulle
May 4, 2020·Current Neuropharmacology·Martina HrabinovaOndrej Soukup

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.

Alzheimer's Disease: APP

Amyloid precursor protein (APP) proteolysis is critical for the development of Alzheimer's disease, a neurodegenerative disease associated with accumulation of amyloid plaques in the brain. Here is the latest research on APP and Alzheimer's disease.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.