Amyloid precursor protein mediated changes in intestinal epithelial phenotype in vitro.

PloS One
Kendra L PuigColin K Combs

Abstract

Although APP and its proteolytic metabolites have been well examined in the central nervous system, there remains limited information of their functions outside of the brain. For example, amyloid precursor protein (APP) and amyloid beta (Aβ) immunoreactivity have both been demonstrated in intestinal epithelial cells. Based upon the critical role of these cells in absorption and secretion, we sought to determine whether APP or its metabolite amyloid β (Aβ), had a definable function in these cells. The human colonic epithelial cell line, Caco-2 cells, were cultured to examine APP expression and Aβ secretion, uptake, and stimulation. Similar to human colonic epithelium stains, Caco-2 cells expressed APP. They also secreted Aβ 1-40 and Aβ 1-42, with LPS stimulating higher concentrations of Aβ 1-40 secretion. The cells also responded to Aβ 1-40 stimulation by increasing IL-6 cytokine secretion and decreasing cholesterol uptake. Conversely, stimulation with a sAPP-derived peptide increased cholesterol uptake. APP was associated with CD36 but not FATP4 in co-IP pull down experiments from the Caco-2 cells. Moreover, stimulation of APP with an agonist antibody acutely decreased CD36-mediated cholesterol uptake. APP exists as part of a m...Continue Reading

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Citations

Mar 7, 2017·Scientific Reports·Kendra L PuigColin K Combs
Mar 26, 2019·Current Alzheimer Research·Kristina Endres
Aug 4, 2018·Frontiers in Microbiology·Bart C WeimerJigna Shah
Dec 31, 2020·International Journal of Molecular Sciences·Yuanpeng ZhengAletta D Kraneveld
Jul 31, 2021·Frontiers in Cell and Developmental Biology·Gaelle NoelMarcela F Pasetti

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Methods Mentioned

BETA
transgenic
ELISAs
electrophoresis
Assay
pull-down
co-immunoprecipitation
ELISA

Software Mentioned

Adobe Photoshop

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