Abstract
Hepatocellular carcinoma (HCC) is a malignant tumor with high morbidity and mortality globally. It accounts for the majority of primary liver cancer cases. Amyloid precursor protein (APP), a cell membrane protein, plays a vital role in the pathogenesis of Alzheimer's disease, and has been found to be implicated in tumor growth and metastasis. Therefore, to understand the relationship between APP and 5-fluorouracil (5-FU) resistance in liver cancer, Cell Counting Kit-8, apoptosis and cell cycle assays, western blotting, and reverse transcription-quantitative polymerase chain reaction (qPCR) analysis were performed. The results demonstrated that APP expression in Bel7402-5-FU cells was significantly up-regulated, as compared with that in Bel7402 cells. Through successful construction of APP-silenced (siAPP) and overexpressed (OE) Bel7402 cell lines, data revealed that the Bel7402-APP751-OE cell line was insensitive, while the Bel7402-siAPP cell line was sensitive to 5-FU in comparison to the matched control group. Furthermore, APP overexpression decreased, while APP silencing increased 5-FU-induced apoptosis in Bel7402 cells. Mechanistically, APP overexpression and silencing can regulate the mitochondrial apoptotic pathway and th...Continue Reading
References
Jun 26, 2001·Oncogene·C A BelmokhtarE Ségal-Bendirdjian
Apr 5, 2002·Molecular Cell·Yigong Shi
Jan 2, 2009·Cancer Research·Ken-ichi TakayamaSatoshi Inoue
Feb 20, 2009·Nature·Anatoly NikolaevMarc Tessier-Lavigne
Aug 25, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Pavlo L KovalenkoMarc D Basson
Sep 12, 2013·The Journal of Biological Chemistry·Neha Kabra Woods, Jaya Padmanabhan
Feb 20, 2014·Oncology Reports·Yong ZhaJing Tan
May 9, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Dara Y KallopRobby M Weimer
Sep 15, 2014·Biochemical and Biophysical Research Communications·Toshiaki MiyazakiSatoshi Inoue
Oct 12, 2014·Biochemical and Biophysical Research Communications·Mallory GoughEdward Parkin
Nov 19, 2014·Annals of Surgical Oncology·Hidenari HirataKoshi Mimori
Apr 9, 2015·World Journal of Gastroenterology : WJG·Myeong Jun Song
Jun 16, 2015·World Journal of Gastroenterology : WJG·Yi-Jun ZhaoJun Yang
Dec 3, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Wei DaiGenhai Shen
Mar 5, 2016·Molecular Medicine Reports·Shihong MaGuangmei Zhang
Jun 16, 2016·Experimental and Molecular Pathology·Jinxia LiuRunzhou Ni
Jul 3, 2016·Molecular Neurobiology·Mar Rodríguez-RuizRafael Franco
Aug 4, 2016·Nature·Boris StrilicStefan Offermanns
Jan 6, 2017·The FEBS Journal·John Hardy
Jan 27, 2017·Neurochemical Research·Xi WangLixin Li
Apr 28, 2017·Oncotarget·Jing ZhangRongkuan Li
Jun 8, 2017·Progress in Neurobiology·Hassan BukhariThorsten Müller
Jul 18, 2017·Acta Pharmacologica Sinica·Jing-Qiu LiZe-Hong Miao
Jul 26, 2017·Journal of Cancer Survivorship : Research and Practice·Helena HarderVal Jenkins
Nov 24, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Ting SunLiang Ming
Dec 19, 2017·Japanese Journal of Clinical Oncology·Masafumi IkedaJunji Furuse
Jan 10, 2018·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Feb 6, 2018·Lancet·Claudia AllemaniUNKNOWN CONCORD Working Group
Feb 28, 2018·Molecular Medicine Reports·Wei WangSuying Yan
Mar 11, 2018·Journal of Cancer Survivorship : Research and Practice·Amanda GehrkeMichael Feuerstein
Mar 17, 2018·Toxicology Letters·María Paula CeballosMaría Cristina Carrillo
Mar 25, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jun LiZongfang Li
Feb 19, 2019·Drug Design, Development and Therapy·Shiyu GaoHaiyan Hu