Amyloidogenic cross-seeding of Tau protein: Transient emergence of structural variants of fibrils

PloS One
Bartosz NizynskiKrzysztof Nieznanski

Abstract

Amyloid aggregates of Tau protein have been implicated in etiology of many neurodegenerative disorders including Alzheimer's disease (AD). When amyloid growth is induced by seeding with preformed fibrils assembled from the same protein, structural characteristics of the seed are usually imprinted in daughter generations of fibrils. This so-called conformational memory effect may be compromised when the seeding involves proteins with non-identical sequences leading to the emergence of distinct structural variants of fibrils (amyloid 'strains'). Here, we investigate cross-seeding of full-length human Tau (FL Tau) with fibrils assembled from K18 and K18ΔK280 fragments of Tau in the presence of poly-L-glutamate (poly-Glu) as an enhancer of Tau aggregation. To study cross-seeding between Tau polypeptides and the role of the conformational memory effect in induction of Tau amyloid polymorphism, kinetic assays, transmission electron microscopy, infrared spectroscopy and limited proteolysis have been employed. The fastest fibrillization was observed for FL Tau monomers seeded with preformed K18 amyloid yielding daughter fibrils with unique trypsin digestion patterns. Morphological features of daughter FL Tau fibrils induced by K18 and ...Continue Reading

References

Jan 5, 1990·Science·B EddéP Denoulet
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C M WischikR A Crowther
May 1, 1997·Developmental Biology·C M HedgepethP S Klein
Nov 21, 1998·The Journal of Biological Chemistry·B CaugheyR A Bessen
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·P FriedhoffE Mandelkow
Feb 24, 2001·Annals of the New York Academy of Sciences·M Hutton
Feb 28, 2001·Progress in Biophysics and Molecular Biology·A Barth
Nov 2, 2002·The EMBO Journal·Marcus Fändrich, Christopher M Dobson
Jun 1, 2004·Protein Science : a Publication of the Protein Society·Wojciech DzwolakRoland Winter
Jun 11, 2004·Annual Review of Biochemistry·Peter ChienAngela H DePace
Mar 8, 2005·Neurobiology of Aging·Angela L Guillozet-BongaartsLester I Binder
Jun 10, 2005·Nature·Christiane RitterRoland Riek
Aug 30, 2005·Journal of Molecular Biology·Kei-Ichi YamaguchiYuji Goto
Jul 1, 2006·Nature·Motomasa TanakaJonathan S Weissman
Jan 6, 2007·Journal of Neuropathology and Experimental Neurology·John C van SwietenPeter Heutink
May 12, 2007·Brain Pathology·Eckhard MandelkowEva-Maria Mandelkow
May 29, 2007·The Journal of Biological Chemistry·Hirofumi AoyagiAkira Tamaoka
Aug 19, 2007·Biochimica Et Biophysica Acta·Andreas Barth
Jan 30, 2008·Current Opinion in Cell Biology·Jennetta W HammondKristen J Verhey
Sep 6, 2008·Biochemistry·Efstratios MylonasDmitri I Svergun
Nov 18, 2008·The Journal of Biological Chemistry·Bess FrostMarc I Diamond
Mar 31, 2009·The Journal of Biological Chemistry·Natallia MakaravaIlia V Baskakov
May 14, 2009·HFSP Journal·Jeppe FalsigAdriano Aguzzi
May 8, 2010·Prion·Rodrigo Diaz-Espinoza, Claudio Soto
Jun 1, 2010·The Journal of Physical Chemistry. B·Aleksandra Fulara, Wojciech Dzwolak
Jun 2, 2011·Topics in Current Chemistry·Witold K Surewicz, Marcin I Apostol
Jun 11, 2011·The Journal of Biological Chemistry·Yoshiaki FurukawaNobuyuki Nukina
Dec 31, 2011·Molecular Pharmaceutics·Tomasz WasiakBarbara Klajnert
Mar 7, 2012·The Journal of Biological Chemistry·Xiang YuBuyong Ma
Mar 20, 2012·Cell·David Eisenberg, Mathias Jucker
Jul 5, 2012·Cold Spring Harbor Perspectives in Medicine·Eva-Maria Mandelkow, Eckhard Mandelkow
Dec 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Susanne WegmannDaniel J Müller
Jul 31, 2013·Proceedings of the National Academy of Sciences of the United States of America·Brandon B HolmesMarc I Diamond

❮ Previous
Next ❯

Citations

Sep 19, 2019·Cellular and Molecular Life Sciences : CMLS·Ye TianZhentao Zhang
Mar 3, 2019·Acta Neuropathologica Communications·Yann FichouIsabelle Landrieu
Jan 11, 2020·Frontiers in Neuroscience·Yann FichouSongi Han
Dec 4, 2019·Frontiers in Molecular Neuroscience·Woo Shik ShinLin Jiang
Oct 21, 2020·Cellular and Molecular Life Sciences : CMLS·Yifan ZengYongqi Huang
Jan 30, 2021·Acta Neuropathologica Communications·Nora Bengoa-VergnioryJavier Alegre-Abarrategui
Jul 12, 2021·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Hanna NieznanskaKrzysztof Nieznanski

❮ Previous
Next ❯

Methods Mentioned

BETA
protein assay
electrophoresis
infrared
electron microscopy
transgenic
fluorescence assay

Software Mentioned

GRAMS

Related Concepts

Related Feeds

Alzheimer's Disease: Genetics

Alzheimer's disease is a neurodegenerative disease. Discover genetic and epigenetic aspects of Alzheimer’s disease, including genetic markers and genomic structural variations with this feed.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Alzheimer's Disease: APP

Amyloid precursor protein (APP) proteolysis is critical for the development of Alzheimer's disease, a neurodegenerative disease associated with accumulation of amyloid plaques in the brain. Here is the latest research on APP and Alzheimer's disease.

Alzheimer's Disease: Tau & TDP-43

Alzheimer's disease is a neurodegenerative disease. This feed focuses on the underlying role of tau proteins and TAR DNA-binding protein 43, as well as other genetic factors, in Alzheimer's disease.

Alzheimer's Disease: Amyloid Beta

Alzheimer's disease is a neurodegenerative disease associated with the accumulation of amyloid plaques in the brain; these plaques are comprised of amyloid beta deposits. Here is the latest research in this field.