An 8-gene mRNA expression profile in circulating tumor cells predicts response to aromatase inhibitors in metastatic breast cancer patients

BMC Cancer
Esther A ReijmStefan Sleijfer

Abstract

Molecular characterization of circulating tumor cells (CTC) is promising for personalized medicine. We aimed to identify a CTC gene expression profile predicting outcome to first-line aromatase inhibitors in metastatic breast cancer (MBC) patients. CTCs were isolated from 78 MBC patients before treatment start. mRNA expression levels of 96 genes were measured by quantitative reverse transcriptase polymerase chain reaction. After applying predefined exclusion criteria based on lack of sufficient RNA quality and/or quantity, the data from 45 patients were used to construct a gene expression profile to predict poor responding patients, defined as disease progression or death <9 months, by a leave-one-out cross validation. Of the 45 patients, 19 were clinically classified as poor responders. To identify them, the 75% most variable genes were used to select genes differentially expressed between good and poor responders. An 8-gene CTC predictor was significantly associated with outcome (Hazard Ratio [HR] 4.40, 95% Confidence Interval [CI]: 2.17-8.92, P < 0.001). This predictor identified poor responding patients with a sensitivity of 63% and a positive predictive value of 75%, while good responding patients were correctly predicted ...Continue Reading

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Citations

Oct 21, 2016·Neoplasia : an International Journal for Oncology Research·Nick BeijeStefan Sleijfer
Nov 2, 2018·Genes, Chromosomes & Cancer·Evi Lianidou, Klaus Pantel
Feb 12, 2019·PLoS Computational Biology·Matthew RuffaloZiv Bar-Joseph
Feb 15, 2019·International Journal of Cancer. Journal International Du Cancer·Ingeborg E de KruijffStefan Sleijfer
Mar 4, 2020·International Journal of Molecular Sciences·Anna FabisiewiczEwa A Grzybowska
Jan 13, 2018·Advanced Drug Delivery Reviews·William L HwangDavid T Miyamoto

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Methods Mentioned

BETA
PCR

Software Mentioned

STATA
TreeView
Cluster
CellSearch®
CellSearch

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