An ABA-binding protein with nucleic acid-binding property

Science in China. Series C, Life Sciences
Z ZhengC Ma

Abstract

Maize cDNA expression library was screened using the antibodies against ABA-binding proteins (ABBP). From 200 000 separated phage plaques, only one cDNA clone coding for a polypeptide was obtained. It shows 60%-65% homology to the genes of nucleic acid-binding proteins in mammalia. However, about 120 cDNA clones coding maize 17s rRNA were unexpectedly obtained. In view of this, the ABBP purified by ABA affinity chromatography and the antibodies derived from the purified ABBP were examined. It is found that rRNA is indeed present in the ABBP. In addition, the obtained antibodies could recognize RNA as well as ABBP. It is suggested that the ABBP have nucleic acid-binding property and can form a complex with rRNA.

References

May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·N InoharaM Futai
Mar 8, 1986·British Dental Journal·P CoventryA C Mellor
Apr 1, 1994·Plant Physiology·B. E. AndersonJ. I. Schroeder
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H Barbier-BrygooJ Guern

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Citations

Jan 1, 2002·The Arabidopsis Book·Ruth R Finkelstein, Christopher D Rock

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