An ABA-responsive element in the AtSUC1 promoter is involved in the regulation of AtSUC1 expression.

Planta
Stefan HothNorbert Sauer

Abstract

Abscisic acid (ABA) and sugars regulate many aspects of plant growth and development, and we are only just beginning to understand the complex interactions between ABA and sugar signaling networks. Here, we show that ABA-dependent transcription factors bind to the promoter of the Arabidopsis thaliana AtSUC1 (At1g71880) sucrose transporter gene in vitro. We present the characterization of a cis-regulatory element by truncation of the AtSUC1 promoter and by electrophoretic mobility shift assays that is identical to a previously characterized ABA-responsive element (ABRE). In yeast 1-hybrid analyses we identified ABI5 (AtbZIP39; At2g36270) and AREB3 (AtbZIP66; At3g56850) as potential interactors. Analyses of plants expressing the beta-glucuronidase reporter gene under the control of ABI5 or AREB3 promoter sequences demonstrated that both transcription factor genes are co-expressed with AtSUC1 in pollen and seedlings, the primary sites of AtSUC1 action. Mutational analyses of the identified cis-regulatory element verified its importance for AtSUC1 expression in young seedlings. In abi5-4 seedlings, we observed an increase of sucrose-dependent anthocyanin accumulation and AtSUC1 mRNA levels. This suggests that ABI5 prevents an overs...Continue Reading

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Citations

Nov 5, 2011·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Eric DuchêneDidier Merdinoglu
Oct 25, 2011·Journal of Experimental Botany·Kathrin Wippel, Norbert Sauer
Sep 30, 2010·Plant Physiology·Seok-Won JeongYoun-Il Park
Apr 25, 2012·Plant Science : an International Journal of Experimental Plant Biology·Kevin N BaronClaudio Stasolla
Apr 25, 2012·Plant Science : an International Journal of Experimental Plant Biology·N GouldP E H Minchin
Nov 3, 2012·Journal of Integrative Plant Biology·Lexiang JiXinmin An
Mar 25, 2017·Plant & Cell Physiology·Jörg HirscheThomas Roitsch
Apr 27, 2021·Trends in Plant Science·Hongfei LiYanxia Zhang
May 27, 2021·Journal of Experimental Botany·Qiyu Xu, Johannes Liesche

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