An ABC transporter and a TonB ortholog contribute to Helicobacter mustelae nickel and cobalt acquisition.

Infection and Immunity
Jeroen StoofArnoud H M van Vliet

Abstract

The genomes of Helicobacter species colonizing the mammalian gastric mucosa (like Helicobacter pylori) contain a large number of genes annotated as iron acquisition genes but only few nickel acquisition genes, which contrasts with the central position of nickel in the urease-mediated acid resistance of these gastric pathogens. In this study we have investigated the predicted iron and nickel acquisition systems of the ferret pathogen Helicobacter mustelae. The expression of the outer membrane protein-encoding frpB2 gene was iron and Fur repressed, whereas the expression of the ABC transporter genes fecD and ceuE was iron and Fur independent. The inactivation of the two tonB genes showed that TonB1 is required for heme utilization, whereas the absence of TonB2 only marginally affected iron-dependent growth but led to reduced cellular nickel content and urease activity. The inactivation of the fecD and ceuE ABC transporter genes did not affect iron levels but resulted in significantly reduced urease activity and cellular nickel content. Surprisingly, the inactivation of the nixA nickel transporter gene affected cellular nickel content and urease activity only when combined with the inactivation of other nickel acquisition genes, l...Continue Reading

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Citations

Jul 14, 2011·Proceedings of the National Academy of Sciences of the United States of America·Bryan W DaviesJohn J Mekalanos
Sep 3, 2011·Environmental Microbiology·Isabelle J SchalkArmelle Braud
Sep 22, 2015·Frontiers in Microbiology·Kathryn P Haley, Jennifer A Gaddy
Oct 1, 2016·Gastroenterology Research and Practice·Kathryn P Haley, Jennifer A Gaddy
Dec 19, 2014·Metallomics : Integrated Biometal Science·M D JonesD B Zamble
Feb 15, 2018·Scientific Reports·Ryo OnoderaNoriyasu Hirasawa
Aug 14, 2012·Metallomics : Integrated Biometal Science·Peter T ChiversJessica L Rowe
Jan 28, 2015·Metallomics : Integrated Biometal Science·Peter T Chivers
Feb 12, 2021·Metallomics : Integrated Biometal Science·Brittni R KelleyJeremiah G Johnson

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