An Accelerated Analytical Process for the Development of STR Profiles for Casework Samples

Journal of Forensic Sciences
Nancy Laurin, Chantal Frégeau

Abstract

Significant efforts are being devoted to the development of methods enabling rapid generation of short tandem repeat (STR) profiles in order to reduce turnaround times for the delivery of human identification results from biological evidence. Some of the proposed solutions are still costly and low throughput. This study describes the optimization of an analytical process enabling the generation of complete STR profiles (single-source or mixed profiles) for human identification in approximately 5 h. This accelerated process uses currently available reagents and standard laboratory equipment. It includes a 30-min lysis step, a 27-min DNA extraction using the Promega Maxwell(®) 16 System, DNA quantification in <1 h using the Qiagen Investigator(®) Quantiplex HYres kit, fast amplification (<26 min) of the loci included in AmpFℓSTR(®) Identifiler(®), and analysis of the profiles on the 3500-series Genetic Analyzer. This combination of fast individual steps produces high-quality profiling results and offers a cost-effective alternative approach to rapid DNA analysis.

References

Dec 17, 2008·Forensic Science International. Genetics·Peter M ValloneJohn M Butler
Feb 5, 2010·Forensic Science International. Genetics·Adrian LinacreShanan S Tobe
May 12, 2010·Forensic Science International. Genetics·Chantal J FrégeauRon M Fourney
Aug 14, 2010·Analytical Chemistry·Andrew J HopwoodGillian Tully
May 3, 2011·Forensic Science International. Genetics·Saskia VerheijTitia Sijen
Jan 24, 2012·Forensic Science International. Genetics·Chantal J Frégeau, Anick De Moors
Aug 28, 2012·Forensic Science International. Genetics·Kathryn OostdikCynthia Sprecher
Aug 7, 2013·Investigative Genetics·Eugene TanRichard F Selden
Sep 23, 2014·Forensic Science International. Genetics·Lori K HennessyStefanie Gangano
Oct 7, 2014·Forensic Science International. Genetics·Mitchell Holland, Frank Wendt
Jan 21, 2015·Forensic Science International. Genetics·Chantal J Frégeau, Nancy Laurin

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Citations

Jan 17, 2016·Forensic Science International : Synergy·Chantal J Frégeau, Claude Dalpé
Nov 4, 2015·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Malur SudhanvaBarnaby Clark
Jan 21, 2015·Forensic Science International. Genetics·Chantal J Frégeau, Nancy Laurin

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