An accurate and inexpensive color-based assay for detecting severe anemia in a limited-resource setting

American Journal of Hematology
Patrick T McGannWilbur A Lam

Abstract

Severe anemia is an important cause of morbidity and mortality among children in resource-poor settings, but laboratory diagnostics are often limited in these locations. To address this need, we developed a simple, inexpensive, and color-based point-of-care (POC) assay to detect severe anemia. The purpose of this study was to evaluate the accuracy of this novel POC assay to detect moderate and severe anemia in a limited-resource setting. The study was a cross-sectional study conducted on children with sickle cell anemia in Luanda, Angola. The hemoglobin concentrations obtained by the POC assay were compared to reference values measured by a calibrated automated hematology analyzer. A total of 86 samples were analyzed (mean hemoglobin concentration 6.6 g/dL). There was a strong correlation between the hemoglobin concentrations obtained by the POC assay and reference values obtained from an automated hematology analyzer (r=0.88, P<0.0001). The POC assay demonstrated excellent reproducibility (r=0.93, P<0.0001) and the reagents appeared to be durable in a tropical setting (r=0.93, P<0.0001). For the detection of severe anemia that may require blood transfusion (hemoglobin <5 g/dL), the POC assay had sensitivity of 88.9% and specif...Continue Reading

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Citations

Dec 14, 2016·Analytical Chemistry·Samiksha NayakSamuel K Sia
Jun 5, 2019·Annals of the New York Academy of Sciences·Ralph D WhiteheadMaria Elena D Jefferds
Apr 22, 2021·Lab on a Chip·Ran AnUmut A Gurkan
Apr 17, 2021·Biophysical Journal·Christopher D BrownFrank A Ferrone
May 17, 2021·British Journal of Haematology·Sophie UyogaKathryn Maitland

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