An accurate and rapid single step protocol for enumeration of cytokine positive T lymphocytes.

Journal of Immunology and Regenerative Medicine
Deepa RajagopalSandrine Vessillier

Abstract

Accurate determination of cellular subsets that secrete particular cytokine(s) is a significant parameter for functional characterization of an immunological response. The present study was conducted to develop a method for simultaneous measurement of intracellular cytokine positive CD4 and CD8 positive T lymphocytes in a single tube, with a no-wash protocol. We report here the development of a simplified, rapid procedure for precise enumeration of cytokine positive T lymphocytes using BD Trucount tubes. This single step protocol for accurate enumeration of cytokine positive T lymphocytes, will allow for better characterization of immune cell phenotype and function.

References

Sep 15, 2000·Journal of Immunological Methods·P PalaP J Openshaw
Apr 28, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Lili WangRichard Stebbings
Feb 7, 2015·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Richard StebbingsJörg Neukammer
Apr 18, 2018·Immunological Reviews·Miro E RaeberOnur Boyman
Dec 5, 2019·International Journal of Molecular Sciences·Shinwan KanyBorna Relja

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