An Accurate In Vitro Assay for High-Throughput Disease Testing of Phytophthora infestans in Potato

Plant Disease
Sanwen HuangEvert Jacobsen

Abstract

An in vitro inoculation assay was developed as a routine disease testing method to study gene-for-gene interactions in the potato (Solanum tuberosum)-Phytophthora infestans pathosystem. The specificity and reliability of the new method was compared with the well-established detached-leaf assay. Four P. infestans isolates were tested for avirulence on a set of R gene differentials using tissue cultured plantlets and detached leaves. Both methods gave identical conclusions on avirulence profiles of all isolates. A population of 93 clones was phenotyped for segregation of two closely linked and functionally distinct genes-R3a and R3b-in the R3 locus. Both methods resulted in phenotypic scorings that were in perfect agreement for all clones. Furthermore, the phenotyping of the population was fully consistent with the genotyping obtained from analysis of molecular markers that flank each gene. This new assay is quick, space-effective, and accurate and can be used for investigation of the qualitative interaction between potato and P. infestans.

References

Nov 10, 2001·Annual Review of Phytopathology·C Gebhardt, J P Valkonen
May 10, 2002·The Plant Journal : for Cell and Molecular Biology·Agim BallvoraChristiane Gebhardt
Jul 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Junqi SongJiming Jiang
Apr 14, 2004·Molecular Plant-microbe Interactions : MPMI·Sanwen HuangEvert Jacobsen
Apr 6, 2005·The Plant Journal : for Cell and Molecular Biology·Sanwen HuangRichard G F Visser

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Citations

May 27, 2015·Molecular Genetics and Genomics : MGG·Zhengqing XieSihai Yang
Nov 1, 2006·Molecular Plant Pathology·Vivianne G A A VleeshouwersSophien Kamoun
Aug 7, 2020·Plant Disease·Hari S KarkiDennis A Halterman
Jul 3, 2021·Nature Microbiology·Jochem BronkhorstJoris Sprakel

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