An accurate in vitro model of the E. coli envelope.

Angewandte Chemie
Luke A CliftonJeremy H Lakey

Abstract

Gram-negative bacteria are an increasingly serious source of antibiotic-resistant infections, partly owing to their characteristic protective envelope. This complex, 20 nm thick barrier includes a highly impermeable, asymmetric bilayer outer membrane (OM), which plays a pivotal role in resisting antibacterial chemotherapy. Nevertheless, the OM molecular structure and its dynamics are poorly understood because the structure is difficult to recreate or study in vitro. The successful formation and characterization of a fully asymmetric model envelope using Langmuir-Blodgett and Langmuir-Schaefer methods is now reported. Neutron reflectivity and isotopic labeling confirmed the expected structure and asymmetry and showed that experiments with antibacterial proteins reproduced published in vivo behavior. By closely recreating natural OM behavior, this model provides a much needed robust system for antibiotic development.

References

Oct 1, 1991·The Journal of Clinical Investigation·R T Ellison, T J Giehl
Feb 12, 1998·Journal of Molecular Biology·S A MooreE N Baker
Feb 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Richard F CollinsJames H Naismith
Feb 20, 2007·The Journal of Physical Chemistry. B·Thomas AbrahamJohn Katsaras
Jan 18, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Arwel V HughesStephen J Roser
Nov 11, 2008·Nature Reviews. Microbiology·Jean-Marie PagèsMathias Winterhalter
May 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Emanuel SchneckMotomu Tanaka
May 27, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Hanna P Wacklin
Nov 15, 2011·The Journal of Biological Chemistry·Luke A CliftonJeremy H Lakey
Mar 6, 2012·International Journal of Antimicrobial Agents·David M Livermore
Oct 18, 2013·Journal of the Royal Society, Interface·Luke A CliftonStephen A Holt
Jul 18, 2014·Journal of the Royal Society, Interface·Arwel V HughesJeremy H Lakey
Dec 10, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Luke A CliftonJeremy H Lakey
Nov 13, 2012·Journal of Chemical Theory and Computation·Thomas J PiggotSyma Khalid

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Citations

Jun 15, 2016·International Journal of Molecular Sciences·Blanca Iglesias-FigueroaQuintín Rascón-Cruz
Aug 6, 2016·Proceedings of the National Academy of Sciences of the United States of America·Wanatchaporn ArunmaneeJeremy H Lakey
Sep 8, 2016·Scientific Reports·Chih-Yun HsiaSusan Daniel
Nov 4, 2016·The Journal of Physical Chemistry. B·Pin-Chia HsuSyma Khalid
Dec 6, 2016·Acta Crystallographica. Section D, Structural Biology·Arwel V HughesJohn R P Webster
Jul 12, 2017·ACS Infectious Diseases·Mei-Ling HanTony Velkov
Jan 4, 2019·Acta Crystallographica. Section D, Structural Biology·Olga B FlorekRichard A Frazier
Feb 20, 2020·Annual Review of Physical Chemistry·Wonpil Im, Syma Khalid
Jul 25, 2018·Proceedings of the National Academy of Sciences of the United States of America·Nicolò ParaciniJeremy H Lakey
Sep 22, 2018·The Journal of Biological Chemistry·Raghavendar Reddy Sanganna GariLukas K Tamm
Jun 27, 2019·Nature Microbiology·Gareth W HughesTimothy J Knowles
Dec 14, 2018·The Biochemical Journal·William A CramerS D Zakharov
Oct 3, 2020·Biochemical Society Transactions·Nicolò ParaciniJeremy H Lakey
Jun 7, 2018·Biophysical Journal·Nathaniel Nelson, Daniel K Schwartz
Mar 25, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Dennis J MichalakDavid P Hoogerheide
Apr 12, 2021·Journal of Colloid and Interface Science·Laura H JohnLuke A Clifton
Apr 16, 2021·Advanced Drug Delivery Reviews·Robert Richter, Claus-Michael Lehr
Jul 29, 2021·The Journal of Physical Chemistry. B·Shokoofeh NourbakhshBae-Yeun Ha
Dec 11, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Shuo QianLuke A Clifton
Sep 26, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Luke Ashley CliftonMaximilian W A Skoda
Oct 19, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Samantha MicciullaEmanuel Schneck
Dec 15, 2016·ACS Applied Materials & Interfaces·Shu J LamGreg G Qiao
Jul 2, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Pradyumn SharmaJaydeep Kumar Basu
May 1, 2020·Molecular Pharmaceutics·Kalliopi-Kelli A VanderaCécile A Dreiss
Jul 7, 2021·ACS Infectious Diseases·Zeinab MohamedSusan Daniel

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Methods Mentioned

BETA
neutron scattering
FRET

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