An acellular cerebellar biological scaffold: Preparation, characterization, biocompatibility and effects on neural stem cells

Brain Research Bulletin
Tongming ZhuJianhong Zhu

Abstract

Biomaterial and regenerative medical research has diversified and developed rapidly. A biological scaffold consisting of an extracellular matrix (ECM) functions not only as a supportive material but also as a regulator of cellular functions. Although decellularized scaffolds have been widely applied for the repair of non-central nervous system (CNS) tissues, their efficacy in the CNS has not been extensively investigated. In this report, we describe a dynamic decellularization protocol that combined intracardial perfusion and a series of treatments to effectively remove the cellular components from the cerebellum, which is a unique and relatively simple CNS structure. The resulting cerebellar scaffold retained neurosupportive proteins and growth factors and, when tested with neural stem cells (NSCs) in vitro, was found to be cytocompatible and to stimulate the proliferation and migration of these cells. NSCs that were cultured in vitro on the scaffold differentiated into neurons and astrocytes, as indicated by their expression of βIII-tubulin and glial fibrillary acidic protein (GFAP). Through subcutaneous and intracranial implantation experiments, this preliminary study demonstrated the in vivo biocompatibility of the cerebell...Continue Reading

References

Jan 1, 1995·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·S KoochekpourG J Pilkington
Sep 29, 1995·Experientia·P Simon-AssmannP Simo
Jan 22, 2000·Brain Research Bulletin·M Ito
Feb 26, 2000·Science·F H Gage
Mar 25, 2000·Urological Research·P P ParnigottoP Midrio
May 29, 2002·Brain Research Bulletin·Arturo Alvarez-BuyllaFiona Doetsch
Sep 25, 2003·Journal of Neurology·H J ten DonkelaarW O Renier
Nov 9, 2004·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Maria Teresa ConconiDomenico Ribatti
Oct 12, 2005·Plastic and Reconstructive Surgery·Charles E ButlerSteven J Kronowitz
Apr 8, 2006·Journal of Biomedical Materials Research. Part a·Sara R ChastainAndrew J Putnam
Jul 11, 2006·Annual Review of Cell and Developmental Biology·Celeste M Nelson, Mina J Bissell
Jan 9, 2007·European Urology·Enzo PalminteriEdoardo Austoni
May 18, 2007·Annual Review of Cell and Developmental Biology·Roy V Sillitoe, Alexandra L Joyner
Jun 2, 2007·The Journal of Bone and Joint Surgery. British Volume·B J R F BollandD G Dunlop
Nov 6, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Wyatt PotterWeiyuan J Kao
May 3, 2008·Cancer Research·Courtney M WilliamsJean E Schwarzbauer
Jul 26, 2008·Tissue Engineering. Part a·Janet E ReingStephen F Badylak
Jun 27, 2009·Science·Dennis E DischerPeter W Zandstra
Mar 23, 2010·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Dalit BarkanAnn F Chambers
May 19, 2010·Developmental Biology·Sarah CalveHans-Georg Simon
Jun 26, 2010·Science·Thomas H PetersenLaura E Niklason
Jul 16, 2010·Nature Medicine·Harald C OttJoseph P Vacanti
Aug 28, 2010·Matrix Biology : Journal of the International Society for Matrix Biology·Ekaterina VorotnikovaSusan J Braunhut
Sep 21, 2010·Brain Research Bulletin·Xi-Tao YangDong-Fu Feng
Nov 10, 2010·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Katlin B MassirerAlysson R Muotri
Feb 8, 2011·Biomaterials·Peter M CrapoStephen F Badylak
Feb 15, 2011·Stem Cells·Slaven ErcegShomi S Bhattacharya
Mar 23, 2011·Annual Review of Biomedical Engineering·Stephen F BadylakKorkut Uygun
Sep 3, 2011·Tissue Engineering. Part a·Jessica A DeQuachKaren L Christman
Sep 8, 2011·Developmental Neurobiology·Ilias Kazanis, Charles ffrench-Constant
Oct 8, 2011·Interactive Cardiovascular and Thoracic Surgery·Andrea QuartiMarco Pozzi
Feb 22, 2012·Biomaterials·Peter M CrapoStephen F Badylak
Mar 13, 2012·Development, Growth & Differentiation·Mikio Hoshino
Nov 20, 2012·Biomaterials·Christopher J MedberryStephen F Badylak
Jan 29, 2013·Biomedical Materials·J E Arenas-HerreraJ J Yoo
Apr 20, 2013·Biomaterials·Fabio Zomer VolpatoPaul D Dalton
Dec 9, 2014·Expert Opinion on Biological Therapy·Glenn D Prestwich, Kevin E Healy

❮ Previous
Next ❯

Citations

Aug 9, 2016·Journal of Biotechnology·Delyan P IvanovAnna M Grabowska
Dec 3, 2016·Neural Regeneration Research·Ting-Gang WangWei-Jiang Wu
Nov 20, 2019·Annals of Biomedical Engineering·Madeline C Cramer, Stephen F Badylak
Aug 10, 2019·Methods : a Companion to Methods in Enzymology·Michael J BuckenmeyerBryan N Brown
Sep 1, 2020·Tissue & Cell·Alessandro E C GranatoMarimelia Porcionatto

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Bladder Carcinoma In Situ

Bladder Carcinoma In Situ is a superficial bladder cancer that occurs on the surface layer of the bladder. Discover the latest research on this precancerous condition in this feed.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Astrocytes in Repair & Regeneration

Astrocytes are glial cells found within the CNS and are able to regenerate new neurons. They become activated during CNS injury and disease. The activation leads to the transcription of new genes and the repair and regeneration of neurons. Discover the latest research on astrocytes in repair and regeneration here.

© 2021 Meta ULC. All rights reserved