PMID: 46634Jan 1, 1975

An acetylcholinesterase method for in toto staining of peripheral nerves

Stain Technology
B Baljet, J Drukker

Abstract

Stomach, small intestine, uterus, urinary bladder, vagina, mesentery, mesometrium and joint capsule of rats, gall bladder, cystic duct and bile duct of dogs and uteri of young children are stained in toto. Tissue is perfused with saline containing hyaluronidase, then pinned on a flat layer of Paraplast and fixed for 24 hr in cold sucrose formol solution. Stomach, urinary bladder and gall bladder are also fixed in toto. Rinse for 2 days in cold 0.22 M sucrose in a sodium cacodylate buffer pH 7.2. Incubate in medium consisting of 60 mM acetate-buffer pH 5.0 or pH 5.6 (for human material only), 2 mM acetylthiocholine iodide, 15 mM Na citrate, 3 mM Cu sulphate, 0.5 mM K3Fe(CN)6, 5 times 10-4 M iso-OMPA, 1% Triton X 100 at 37C. Rinse in doubly distilled water. Dehydrate in glycerine/water mixtures of increasing glycerine content. Store in glycerine or delaminate under dissecting microscope. Delaminated specimens are mounted on gelatinized object glasses, cleared in xylene and coverslipped with Malinol. Specimens stored in glycerine can be studied microscopically. Stained specimens can also be embedded in Paraplast and sections can be studied after counterstaining.

References

Jan 1, 1981·Anatomy and Embryology·D W Hamer, R M Santer
Jan 1, 1987·Anatomy and Embryology·G J GroenJ Drukker
Jan 1, 1988·Anatomy and Embryology·K KaehnR E Poelmann
Jan 1, 1988·Acta neurochirurgica·G J GroenJ Drukker
Sep 1, 1987·Journal of Neuroscience Methods·E A HeinickeJ Wijsman
May 1, 1994·Journal of the Autonomic Nervous System·P A Steele, J K Choate
Feb 1, 1993·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·C Cracco, G Filogamo
Aug 1, 1989·Documenta Ophthalmologica. Advances in Ophthalmology·B BaljetA J Otto
Jan 1, 1987·Clinical and Experimental Hypertension. Part A, Theory and Practice·J DrukkerB Baljet
Jul 24, 2007·Experimental Physiology·Eric K A CorbettTrevor F C Batten
Jan 1, 1979·Journal of Biomechanics·H van Mameren, J Drukker
Dec 19, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C J Van GinnekenA Weyns
Feb 19, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J RiceR P Kapur
Jul 1, 1990·The American Journal of Anatomy·G J GroenJ Drukker
Apr 20, 2001·Laboratory Investigation; a Journal of Technical Methods and Pathology·H TakayamaG Merlino

Citations

Apr 1, 1954·The Anatomical Record·W PALLIEG WEDDELL
Mar 1, 1964·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M J KARNOVSKY, L ROOTS

Related Concepts

Joint Capsule
1,1'-(4,4,7,7-tetramethyl-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-thiazole)-2-methylidene)quinolinium
Glycerin
Tetraisopropylpyrophosphamide
Entire Cystic Duct
Neoplasm of Uncertain or Unknown Behavior of Uterus
Uterus
Ache
Small Intestinal Wall Tissue
Xylene

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