PMID: 37083May 15, 1979

An acid protease in human erythrocytes and its localization in the inner membrane

European Journal of Biochemistry
T MurakamiT Murachi

Abstract

The isolation of erythrocytes of high purity from human blood was achieved by a combination of the two well established methods cells in erythrocyte preparations of different purities was studied. The acid protease activity was recovered to a level comparable with the recovery of erythrocytes, while the neutral protease activity as detected by the release of acid-soluble peptides from hemoglobin or casein disappeared in proportion to the removal of white blood cells. An acid protease was solubilized from the membranes of the purified erythrocytes by the extraction with 1-butanol. The enzyme was active in a pH range from 2 to 4, and sensitive to pepstatin. It was named pH-3 protease after its pH optimum. Sealed ghosts with right-side-out membranes and inside-out vesicles with reverted membranes were prepared from the purified erythrocytes and compared with respect to pH-3 protease activity for its latency as well as its inactivation by tryptic digestion. The results obtained indicate that pH-3 protase is localized on the inner surface of erythrocyte membranes. The self-digestion experiments at pH 4 using the sealed ghosts showed higher availability to pH-3 protease of spectrin and IVa protein than the other membrane proteins, al...Continue Reading

References

Jan 1, 1982·The International Journal of Biochemistry·A K LimR Scott
Jan 1, 1984·The International Journal of Biochemistry·S Takehara, H Terayama
Jan 1, 1985·The International Journal of Biochemistry·M GaczyńskaM Soszyński
Jul 1, 1983·Molecular and Biochemical Parasitology·I W Sherman, L Tanigoshi
Jan 1, 1990·Vox Sanguinis·V de AngelisL Vettore
Sep 1, 1980·European Journal of Biochemistry·S PontremoliA De Flora
Jan 1, 1982·European Journal of Biochemistry·A M Golovtchenko-MatsumotoT Osawa
Oct 15, 1982·Archives of Biochemistry and Biophysics·E MelloniS Pontremoli
Jun 1, 1981·Biochemical and Biophysical Research Communications·D A Schafer, D E Hultquist
Jun 29, 2010·Electrophoresis·Roy B LefkowitzMichael J Heller

Citations

Mar 21, 1974·Biochimica Et Biophysica Acta·D ReicheltR J Haschen
Nov 21, 1973·Nature: New Biology·M NakatoT Kankura
Feb 1, 1970·British Journal of Haematology·R Harris, E O Ukaejiofo
Jul 15, 1970·Biochimica Et Biophysica Acta·G L MooreS L Robinson
Jan 1, 1963·Archives of Biochemistry and Biophysics·J T DODGED J HANAHAN
Sep 20, 1938·The Journal of General Physiology·M L Anson
Dec 1, 1972·The Journal of Membrane Biology·R J Bernacki, H B Bosmann

Related Concepts

Endopeptidases
Leukocytes
Red Cell Ghost
Erythrocytes
Hydrogen-Ion Concentration
Trypure

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.