An acidified thermostabilizing mini-peptide derived from the carboxyl extension of the larger isoform of the plant Rubisco activase

Journal of Biotechnology
Mengru ZhangZhurong Zou

Abstract

Thermostable fusion peptide partners are valuable in engineering thermostability in proteins. We evaluated the Arabidopsis counterpart (AtRAce) and an acidified derivative (mRAce) of the conserved carboxyl extension (RAce) of plant Rubisco activase (RCA) for their thermostabilizing properties in Escherichia coli and Saccharomyces cerevisiae using a protein fusion strategy. We used AtRAce and mRAce as fusion tails for the thermolabile protein RCA2 from Arabidopsis thaliana and Nicotiana tabacum. The homologous fusion of AtRAce with Arabidopsis RCA2 and the heterologous fusion of AtRAce with tobacco RCA2 increased the thermostability of both proteins. The acidified derivative mRAce conferred greater thermostability upon both proteins as compared with AtRAce. Moreover, mRAce enhanced the thermostability of other two thermolabile proteins from Jatropha curcas: the cytosolic ascorbate peroxidase 1 (JcAPX1) and the TATA-box binding protein isoform 1 (JcTBP1). We further report - for the first time - that JcTBP1 mediates heat tolerance in vivo in yeast. Thus, our study identifies a C-terminal acidic mini-peptide - the acidified derivative mRAce - with potential uses in improving the thermostability of heat-labile proteins and their as...Continue Reading

References

Aug 1, 1992·Trends in Genetics : TIG·R J White, S P Jackson
Nov 9, 2000·Proceedings of the National Academy of Sciences of the United States of America·S J Crafts-Brandner, M E Salvucci
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ning ZhangArchie R Portis
Jul 25, 2003·Trends in Biochemical Sciences·Irwin Davidson
Apr 7, 2004·Protein Engineering, Design & Selection : PEDS·Sang Myun ParkJongsun Kim
Apr 18, 2006·Biochemical and Biophysical Research Communications·He HuangArio de Marco
Aug 2, 2007·Journal of the American Chemical Society·Michael S LawrenceDavid R Liu
Aug 9, 2007·The FEBS Journal·Kathryn A LukePernilla Wittung-Stafshede
Oct 15, 2008·The Journal of Biological Chemistry·Shai KoussevitzkyRon Mittler
Feb 23, 2010·Current Opinion in Microbiology·Dominique Böttcher, Uwe T Bornscheuer
Jun 1, 2007·American Journal of Botany·David J WestonWm Vance Baird
Jun 28, 2011·Protein Expression and Purification·Zhurong ZouChunyi Zhang
May 21, 2013·Current Opinion in Structural Biology·Hein J WijmaDick B Janssen
Nov 12, 2013·Biotechnology Advances·Haoran Yu, He Huang

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Citations

Jun 28, 2020·The Plant Journal : for Cell and Molecular Biology·Sang Yeol KimDonald R Ort
Jul 28, 2020·Journal of Experimental Botany·Johanna StockHans-Peter Mock
Sep 12, 2020·Journal of Experimental Botany·Jie YeYuyang Zhang

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