An Acromegaly Disease Zebrafish Model Reveals Decline in Body Stem Cell Number along with Signs of Premature Aging

Biology
Abdalla ElbialyShigeharu Kinoshita

Abstract

In our previous publication, it was shown that growth hormone (GH) excess in acromegaly affects the cell integrity of somatic cells through increased DNA damage throughout the body and impaired DNA repair pathways. Acromegaly is a hormone disorder pathological condition that develops as a result of growth hormone over-secretion from the pituitary gland. We produced a zebrafish acromegaly model to gain a better understanding of the excess GH effects at the cellular level. Here we show that the acromegaly zebrafish model progressively reduced the number of stem cells in different organs and increased oxidative stress in stem cells. Importantly, the decline in the stem cells was even more apparent than in aged fish. The controversy and debate over the use of GH as an anti-aging therapy have been going on for several years. In this study, excess GH induced aging signs such as increased senescence-associated (SA)-β-galactosidase staining of abdominal skin and similarity of the pattern of gene expression between aged and acromegaly zebrafish. Thus, this study highlights the role of excess GH in acromegaly stem cells.

References

Jul 5, 1990·The New England Journal of Medicine·D RudmanD E Mattson
Apr 30, 1998·Physiological Reviews·K B Beckman, B N Ames
Jul 12, 2003·Experimental Gerontology·Shuji KishiStephanie B Tsai
Oct 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aravind SubramanianJill P Mesirov
Nov 30, 2006·Developmental and Comparative Immunology·Isao KobayashiTeruyuki Nakanishi
Jun 28, 2007·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Miriam G San FrutosFranco Sánchez-Franco
Jun 27, 2008·Orphanet Journal of Rare Diseases·Philippe Chanson, Sylvie Salenave
Jan 16, 2009·BMC Genomics·Damian SmedleyArek Kasprzyk
Dec 29, 2009·Free Radical Biology & Medicine·Adam B SalmonViviana I Pérez
Feb 22, 2011·Naunyn-Schmiedeberg's Archives of Pharmacology·Emilie LauresserguesPatrick Duriez
May 20, 2014·Journal of Visualized Experiments : JoVE·Jacob Michael FroehlichPeggy R Biga
May 28, 2014·Interdisciplinary Topics in Gerontology·Yahui KongHong Zhang
Dec 31, 2014·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Esra HatipogluSadi Gundogdu
Apr 29, 2016·Nature Protocols·Ondrej SvobodaPetr Bartunek
Jul 2, 2017·Clinical Science·Young-Mi Go, Dean P Jones
Oct 20, 2018·Biology·Abdalla ElbialyShigeharu Kinoshita
Mar 23, 2019·Nature Reviews. Disease Primers·Annamaria ColaoRosario Pivonello

❮ Previous
Next ❯

Citations

Apr 15, 2021·Open Biology·Abdalla Elbialy

❮ Previous
Next ❯

Methods Mentioned

BETA
Fluorescence-activated cell sorting
flow cytometry
RNA-seq
FACS
RNA Assay
Illumina sequencing
protein Assay
dissection
Assay

Software Mentioned

Fiji
cummerbund
Cufflinks
ENRICHR
cuffdiff
Biomart
Cytobank
Ensembl
GSEA
HISAT2

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Pediatric Endocrinology
A LiuzziP G Chiodini
Expert Opinion on Pharmacotherapy
Sylvère Störmann, Jochen Schopohl
Current Therapy in Endocrinology and Metabolism
G Sassolas
© 2022 Meta ULC. All rights reserved