An actin-depolymerizing factor from the halophyte smooth cordgrass, Spartina alterniflora (SaADF2), is superior to its rice homolog (OsADF2) in conferring drought and salt tolerance when constitutively overexpressed in rice

Plant Biotechnology Journal
Sonali SenguptaNiranjan Baisakh

Abstract

Actin-depolymerizing factors (ADFs) maintain the cellular actin network dynamics by regulating severing and disassembly of actin filaments in response to environmental cues. An ADF isolated from a monocot halophyte, Spartina alterniflora (SaADF2), imparted significantly higher level of drought and salinity tolerance when expressed in rice than its rice homologue OsADF2. SaADF2 differs from OsADF2 by a few amino acid residues, including a substitution in the regulatory phosphorylation site serine-6, which accounted for its weak interaction with OsCDPK6 (calcium-dependent protein kinase), thus resulting in an increased efficacy of SaADF2 and enhanced cellular actin dynamics. SaADF2 overexpression preserved the actin filament organization better in rice protoplasts under desiccation stress. The predicted tertiary structure of SaADF2 showed a longer F-loop than OsADF2 that could have contributed to higher actin-binding affinity and rapid F-actin depolymerization in vitro by SaADF2. Rice transgenics constitutively overexpressing SaADF2 (SaADF2-OE) showed better growth, relative water content, and photosynthetic and agronomic yield under drought conditions than wild-type (WT) and OsADF2 overexpressers (OsADF2-OE). SaADF2-OE preserved...Continue Reading

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Citations

Jan 15, 2021·Current Opinion in Plant Biology·Showkat Ahmad GanieGoetz Hensel
Mar 28, 2021·Plant Physiology and Biochemistry : PPB·Nicolle Louise Ferreira BarrosCláudia Regina Batista de Souza
May 24, 2021·Current Microbiology·Mingyuan Li Mihray Mutallip

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Methods Mentioned

BETA
environmental stress
immunoprecipitation
transgenic
phosphotransferase
transgenics
nucleotide exchange
scanning electron microscopy
reverse
PCR

Software Mentioned

STRING
ACT
TASSER
UCSF Chimera
CLC workbench
LOMETS
RiceNet
Excel
XLSTAT

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