An actin filament population defined by the tropomyosin Tpm3.1 regulates glucose uptake

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Anthony J KeeEdna C Hardeman

Abstract

Actin has an ill-defined role in the trafficking of GLUT4 glucose transporter vesicles to the plasma membrane (PM). We have identified novel actin filaments defined by the tropomyosin Tpm3.1 at glucose uptake sites in white adipose tissue (WAT) and skeletal muscle. In Tpm 3.1-overexpressing mice, insulin-stimulated glucose uptake was increased; while Tpm3.1-null mice they were more sensitive to the impact of high-fat diet on glucose uptake. Inhibition of Tpm3.1 function in 3T3-L1 adipocytes abrogates insulin-stimulated GLUT4 translocation and glucose uptake. In WAT, the amount of filamentous actin is determined by Tpm3.1 levels and is paralleled by changes in exocyst component (sec8) and Myo1c levels. In adipocytes, Tpm3.1 localizes with MyoIIA, but not Myo1c, and it inhibits Myo1c binding to actin. We propose that Tpm3.1 determines the amount of cortical actin that can engage MyoIIA and generate contractile force, and in parallel limits the interaction of Myo1c with actin filaments. The balance between these actin filament populations may determine the efficiency of movement and/or fusion of GLUT4 vesicles with the PM.

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Citations

Feb 6, 2016·Scientific Reports·Nikolas HundtDietmar J Manstein
Apr 27, 2016·Current Biology : CB·Simon BrayfordPeter W Gunning
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Datasets Mentioned

BETA
GSE25013

Methods Mentioned

BETA
acetylation
transgenic
motility assay
FCS
motility assays

Software Mentioned

Molecular Imager Chemi ‐ doc XRS
‐ Spring GX
MTrackJ
Gene
GraphPad Prism
ImageJ

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