An Activatable AIEgen Probe for High-Fidelity Monitoring of Overexpressed Tumor Enzyme Activity and Its Application to Surgical Tumor Excision.

Angewandte Chemie
Haidong LiJuyoung Yoon

Abstract

Monitoring fluctuations in enzyme overexpression facilitates early tumor detection and excision. An AIEgen probe (DQM-ALP) for the imaging of alkaline phosphatase (ALP) activity was synthesized. The probe consists of a quinoline-malononitrile (QM) core decorated with hydrophilic phosphate groups as ALP-recognition units. The rapid liberation of DQM-OH aggregates in the presence of ALP resulted in aggregation-induced fluorescence. The up-regulation of ALP expression in tumor cells was imaged using DQM-ALP. The probe permeated into 3D cervical and liver tumor spheroids for imaging spatially heterogeneous ALP activity with high spatial resolution on a two-photon microscopy platform, providing the fluorescence-guided recognition of sub-millimeter tumorigenesis. DQM-ALP enabled differentiation between tumor and normal tissue ex vivo and in vivo, suggesting that the probe may serve as a powerful tool to assist surgeons during tumor resection.

References

Aug 15, 1983·International Journal of Cancer. Journal International Du Cancer·I W McDickenP M Johnson
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B L Vallee, D S Auld
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·M TsujiiR N DuBois
Mar 8, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Aus Tariq AliNigel J Crowther
Jul 30, 2011·Chemical Society Reviews·Yuning HongBen Zhong Tang
Nov 26, 2011·Science Translational Medicine·Yasuteru UranoHisataka Kobayashi
Nov 10, 2013·Journal of the American Chemical Society·Hua ZhangXiaojun Peng
Jul 1, 2014·Advanced Materials·Ju MeiBen Zhong Tang
Sep 27, 2014·Chemical Reviews·Liang ChengZhuang Liu
Nov 7, 2014·Chemical Society Reviews·Ryan T K KwokBen Zhong Tang
May 6, 2015·Chemical Reviews·Hwan Myung Kim, Bong Rae Cho
Oct 23, 2015·Chemical Reviews·Ju MeiBen Zhong Tang
Nov 16, 2016·Biotechnology Advances·Elisabete C CostaIlídio J Correia
Mar 30, 2017·Biosensors & Bioelectronics·Haidong LiXiaojun Peng
Jan 10, 2018·Angewandte Chemie·Qingqing MiaoKanyi Pu
Aug 23, 2019·Accounts of Chemical Research·Dong Wang, Ben Zhong Tang

❮ Previous
Next ❯

Citations

Oct 8, 2020·Topics in Current Chemistry·Juliang YangFan Xia
Oct 28, 2020·Chemical Communications : Chem Comm·Jintao ZhaoJianguo Fang
Jan 1, 2021·Frontiers in Chemistry·Qing LuXiaoqiang Yu
Feb 26, 2021·Chemical Communications : Chem Comm·Yanqing LiShaohua Wei
Jan 5, 2021·Chembiochem : a European Journal of Chemical Biology·Yandong DouKui Du
Apr 20, 2021·Methods and Applications in Fluorescence·Asanka Amith RajapakshaGuang-Fu Yang
Jun 25, 2021·Nature Communications·Chenxu YanWei-Hong Zhu
Jul 8, 2021·Chemical Society Reviews·Hai-Hao HanTony D James
Aug 21, 2021·Advanced Healthcare Materials·Jun DaiShixuan Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.